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Literature summary for 3.6.1.1 extracted from
- Purification and characterization of inorganic pyrophosphatase from Methanobacterium thermoautotrophicum (strain H) (1994), Biochim. Biophys. Acta, 1206, 231-239.
General Stability
| General Stability | Organism |
|---|---|
| decreasing activity at 4°C in reaction mixture lacking diphosphate , KCl prevents from inactivation, Mg-diphosphate, does not protect from inactivation | Methanothermobacter thermautotrophicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KF | 50% at 0.9 mM | Methanothermobacter thermautotrophicus |  |
| Mn2+ | 60% inhibition at 1 mM Mn2+: 24 mM Mg2+ | Methanothermobacter thermautotrophicus | |
| NaF | - |
Methanothermobacter thermautotrophicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
diphosphate | - |
Methanothermobacter thermautotrophicus | |
| 4.9 | - |
Mg2+ | - |
Methanothermobacter thermautotrophicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+, 15% | Methanothermobacter thermautotrophicus | |
| Mg2+ | required for activity | Methanothermobacter thermautotrophicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
| 100000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | Methanothermobacter thermautotrophicus | - |
2 phosphate | - |
? | |
| diphosphate + H2O | Methanothermobacter thermautotrophicus H | - |
2 phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanothermobacter thermautotrophicus | - |
- |
- |
| Methanothermobacter thermautotrophicus H | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermobacter thermautotrophicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1233 | - |
- |
Methanothermobacter thermautotrophicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl, one year, negligible loss of activity | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + H2O | - |
Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
| diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus | 2 phosphate | - |
? | |
| diphosphate + H2O | - |
Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? | |
| diphosphate + H2O | high specificity | Methanothermobacter thermautotrophicus H | 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25000, gel filtration, dimeric form also active | Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
60°C | Methanothermobacter thermautotrophicus |
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