Protein Variants | Comment | Organism |
---|---|---|
H110Q | catalytic activity unchanged compared to wild type enzyme | Escherichia coli |
H119Q | catalytic activity unchanged compared to wild type enzyme | Escherichia coli |
H136Q | variant can be dissociated in trimers, hydrolytic activity 225% of wild-type enzyme | Escherichia coli |
H140Q | variant can be dissociated in trimers, hydrolytic activity 110% of wild-type enzyme | Escherichia coli |
H60Q | catalytic activity unchanged compared to wild type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
Mg-diphosphate | pH 7.2, hexameric, H136Q enzyme | Escherichia coli | |
0.0027 | - |
Mg-diphosphate | pH 7.2, H140Q enzyme, hexameric | Escherichia coli | |
0.0034 | - |
Mg-diphosphate | pH 8, wild type enzyme | Escherichia coli | |
0.008 | - |
Mg-diphosphate | pH 8, hexameric, H136Q enzyme | Escherichia coli | |
0.198 | - |
Mg-diphosphate | pH 7.2, H136Q enzyme, trimeric | Escherichia coli | |
0.44 | - |
Mg-diphosphate | pH 8, H140Q enzyme, trimeric | Escherichia coli | |
4.2 | - |
Mg-diphosphate | pH 7.2, H140Q enzyme, trimeric | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | four Mg2+ ions per active site required for catalysis | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
6 * 20000, homohexameric, 175 amino acid residues per subunit, wild type enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Escherichia coli | - |
2 phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Escherichia coli | 2 phosphate | - |
r | |
diphosphate + H2O | diphosphate in form of Mg-diphosphate | Escherichia coli | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 20000, homohexameric, 175 amino acid residues per subunit, wild type enzyme | Escherichia coli |
trimer | dissociated variant, SDS-PAGE | Escherichia coli |