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Literature summary for 3.6.1.1 extracted from

  • Krishnan, V.A.; Gnanam, A.
    Properties and regulation of Mg2+-dependent chloroplast inorganic pyrophosphatase from Sorghum vulgare leaves (1988), Arch. Biochem. Biophys., 260, 277-284.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Sorghum bicolor
Cd2+
-
Sorghum bicolor
Co2+
-
Sorghum bicolor
EDTA
-
Sorghum bicolor
L-malate allosteric mechanism Sorghum bicolor
Mn2+
-
Sorghum bicolor
Zn2+
-
Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
diphosphate
-
Sorghum bicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast from mesophyll Sorghum bicolor 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no other divalent cations can substitute for Mg2+ Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration, SDS-PAGE Sorghum bicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Sorghum bicolor
-
2 phosphate
-
?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
478
-
-
Sorghum bicolor

Storage Stability

Storage Stability Organism
0-4°C, (NH4)2SO4, 65% saturated, a few months, activity retained Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose-1-phosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor D-glucose + phosphate
-
?
diphosphate + H2O
-
Sorghum bicolor 2 phosphate
-
?
diphosphate + H2O high specificity Sorghum bicolor 2 phosphate
-
?
tetrapolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ?
-
?
tripolyphosphate + H2O very weak substrate compared to diphosphate Sorghum bicolor ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000 Sorghum bicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
25 stable to freezing, 0°C and thawing 25° Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Sorghum bicolor