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Literature summary for 3.5.99.6 extracted from

  • Lara-Lemus, R.; LIbreros-Minotta, C.A.; Altamirano, M.M.; Calcagno, M.L.
    Purification and characterization of glucosamine-6-phosphate deaminase from dog kidney cortex (1992), Arch. Biochem. Biophys., 297, 213-220.
    View publication on PubMed
Search for more literature for 3.5.99.6

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by glucose 6-phosphate Canis lupus familiaris
N-acetylglucosamine 6-phosphate the cooperativity of the reaction is completely abolished by saturating concentrations of GlcNAcGP, this allosteric modulator activated the reaction with a typical K-effect, overview Canis lupus familiaris

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-2-amino-D-glucitol 6-phosphate
-
 Canis lupus familiaris
additional information no effect by glucose 6-phosphate Canis lupus familiaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the enzyme displays positive homotropic cooperativity towards D-glucosamine 6-phosphate Canis lupus familiaris
0.25
-
 D-glucosamine 6-phosphate hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30400
-
 6 * 30400, SDS-PAGE Canis lupus familiaris
180000
-
 about, gel filtration Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucosamine 6-phosphate + H2O Canis lupus familiaris
-
 D-fructose 6-phosphate + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 1390fold from kidney cortex by affinity chromatography on N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose and on phosphocellulose, the latter behaving as an active site affinity resin Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
85
-
 purified enzyme Canis lupus familiaris

Storage Stability

Storage Stability Organism
4°C, purified enzyme, pH 5.0-9.0, overnight, in presence of different salts, stable Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucosamine 6-phosphate + H2O
-
 Canis lupus familiaris D-fructose 6-phosphate + NH3
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 30400, SDS-PAGE Canis lupus familiaris

Synonyms

Synonyms Comment Organism
More formerly EC 5.3.1.10 Canis lupus familiaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42
-
 D-glucosamine 6-phosphate per subunit, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 Canis lupus familiaris
255
-
 D-glucosamine 6-phosphate hexameric enzyme, hyperbolic kinetic conditions, in presence of 1 mM N-acetylglucosamine 6-phosphate, pH 8.8 Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 assay at Canis lupus familiaris

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH-profile, overview Canis lupus familiaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 4°C, purified enzyme, overnight, in presence of different salts, stable Canis lupus familiaris

pI Value

Organism Comment pI Value Maximum pI Value
Canis lupus familiaris isoelectric focusing and chromatofocusing
-
 5.7