Protein Variants | Comment | Organism |
---|---|---|
C118S/C228S/C239S | decrease in kcat-value, no modification of allosteric activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-2-amino-D-glucitol 6-phosphate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
D-glucosamine 6-phosphate | mutant C118S/C228S/C239S, pH 7.5, 30°C | Escherichia coli | |
0.49 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C | Escherichia coli | |
0.5 | - |
D-glucosamine 6-phosphate | wild-type, pH 7.5, 30°C | Escherichia coli | |
0.51 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C | Escherichia coli | |
0.52 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C164, pH 7.5, 30°C | Escherichia coli | |
0.52 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C206, pH 7.5, 30°C | Escherichia coli | |
4.6 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C206, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli | |
5.1 | - |
D-glucosamine 6-phosphate | mutant C118S/C228S/C239S, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli | |
5.1 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C164, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli | |
5.1 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli | |
5.2 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli | |
5.2 | - |
D-glucosamine 6-phosphate | wild-type, pH 7.5, 30°C, calculated from Hill-equation in absence of allosteric activator | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A759 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucosamine 6-phosphate + H2O | - |
Escherichia coli | D-fructose 6-phosphate + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C164, pH 7.5, 30°C | Escherichia coli | |
69.5 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C206, pH 7.5, 30°C | Escherichia coli | |
70.6 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C | Escherichia coli | |
70.9 | - |
D-glucosamine 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C | Escherichia coli | |
71.6 | - |
D-glucosamine 6-phosphate | wild-type, modified with bimane at C164, pH 7.5, 30°C | Escherichia coli | |
96 | - |
D-glucosamine 6-phosphate | mutant C118S/C228S/C239S, pH 7.5, 30°C | Escherichia coli | |
153 | - |
D-glucosamine 6-phosphate | wild-type, pH 7.5, 30°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00223 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | wild-type, pH 7.5, 30°C | Escherichia coli | |
0.0035 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | wild-type, modified with bimane at C206, pH 7.5, 30°C | Escherichia coli | |
0.0036 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | wild-type, modified with bimane at C164, pH 7.5, 30°C | Escherichia coli | |
0.0038 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C206, pH 7.5, 30°C | Escherichia coli | |
0.0041 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | wild-type, modified with dansyl-aminoethyl moiety at C164, pH 7.5, 30°C | Escherichia coli | |
0.0043 | - |
2-deoxy-2-amino-D-glucitol 6-phosphate | mutant C118S/C228S/C239S, pH 7.5, 30°C | Escherichia coli |