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Literature summary for 3.5.99.2 extracted from
- Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II (2005), Biochemistry, 44, 2319-2329.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(D3) | Bacillus subtilis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P25052 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also catalyses the reaction of EC 2.7.4.7 | Bacillus subtilis | ? | - |
? |  |
| thiamine + H2O | - |
Bacillus subtilis | 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x-ray crystallography | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TenA | - |
Bacillus subtilis |
| thiaminase II | - |
Bacillus subtilis |
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Release 2023.1 (January 2023)
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