Cloned (Comment) | Organism |
---|---|
heterologously overproduced in Escherichia coli with a C-terminal His6-tag | Methanosarcina barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Methanosarcina barkeri |
318700 | - |
x * 318700, calculated from sequence | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | Q46A53 | - |
- |
Methanosarcina barkeri DSM 804 | Q46A53 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | 3 | pH 8.0, 37°C, extract from Escherichia coli cells carrying the expression vector | Methanosarcina barkeri |
66 | - |
pH 8.0, 37°C, purified enzyme, recombinant | Methanosarcina barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | the bifunctional enzyme also shows N5,N10-methylene-H4F dehydrogenase activity, EC 1.5.1.15 | Methanosarcina barkeri | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | the bifunctional enzyme also shows N5,N10-methylene-H4F dehydrogenase activity, EC 1.5.1.15 | Methanosarcina barkeri DSM 804 | 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Methanosarcina barkeri |
? | x * 318700, calculated from sequence | Methanosarcina barkeri |
Synonyms | Comment | Organism |
---|---|---|
bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase | bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 | Methanosarcina barkeri |
FolD | bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 | Methanosarcina barkeri |
methenyl-H4F cyclohydrolase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanosarcina barkeri |