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Literature summary for 3.5.4.9 extracted from

  • Buchenau, B.; Thauer, R.K.
    Tetrahydrofolate-specific enzymes in Methanosarcina barkeri and growth dependence of this methanogenic archaeon on folic acid or p-aminobenzoic acid (2004), Arch. Microbiol., 182, 313-325.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologously overproduced in Escherichia coli with a C-terminal His6-tag Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
x * 31000, SDS-PAGE Methanosarcina barkeri
318700
-
x * 318700, calculated from sequence Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri Q46A53
-
-
Methanosarcina barkeri DSM 804 Q46A53
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2 3 pH 8.0, 37°C, extract from Escherichia coli cells carrying the expression vector Methanosarcina barkeri
66
-
pH 8.0, 37°C, purified enzyme, recombinant Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O the bifunctional enzyme also shows N5,N10-methylene-H4F dehydrogenase activity, EC 1.5.1.15 Methanosarcina barkeri 10-formyltetrahydrofolate
-
?
5,10-methenyltetrahydrofolate + H2O the bifunctional enzyme also shows N5,N10-methylene-H4F dehydrogenase activity, EC 1.5.1.15 Methanosarcina barkeri DSM 804 10-formyltetrahydrofolate
-
?

Subunits

Subunits Comment Organism
? x * 31000, SDS-PAGE Methanosarcina barkeri
? x * 318700, calculated from sequence Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 Methanosarcina barkeri
FolD bifunctional enzyme EC 1.5.1.15/EC 3.5.4.9 Methanosarcina barkeri
methenyl-H4F cyclohydrolase
-
Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Methanosarcina barkeri