Cloned (Comment) | Organism |
---|---|
hexa-His tagged DHCH1 is expressed in Escherichia coli, production of a genetic knock-out | Leishmania major |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania major | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | Leishmania major | - |
10-formyltetrahydrofolate | - |
r | |
additional information | Leishmania major | dehydrogenase and cyclohydrogenase activities are contained in the single bifunctional protein DHCH1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant DHCH1 protein is purified by metal-affinity chromatography | Leishmania major |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.3 | - |
cyclohydrolase activity is monitored by following the consumption of 5,10-methylenetetrahydrofolate yielding 10-formyltetrahydrofolate | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methenyltetrahydrofolate + H2O | - |
Leishmania major | 10-formyltetrahydrofolate | - |
r | |
additional information | dehydrogenase and cyclohydrogenase activities are contained in the single bifunctional protein DHCH1 | Leishmania major | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase | - |
Leishmania major |
DHCH1 | - |
Leishmania major |
methylene tetrahydrofolate dehydrogenase/cyclohydrolase | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Leishmania major |