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Literature summary for 3.5.4.9 extracted from
- A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1 (1999), Eur. J. Biochem., 261, 475-480.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
5,10-methenyltetrahydrofolate | - |
Methylorubrum extorquens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
2 * 22000, SDS-PAGE | Methylorubrum extorquens |
| 45000 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | Methylorubrum extorquens | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | - |
AM1, aerobic methylotrophic proteobacterium, monofunctional enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methylorubrum extorquens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 104 | - |
- |
Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | - |
Methylorubrum extorquens | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 10-formyltetrahydrofolate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 22000, SDS-PAGE | Methylorubrum extorquens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methenylH4F cyclohydrolase | - |
Methylorubrum extorquens |
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Release 2023.1 (January 2023)
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