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Literature summary for 3.5.4.9 extracted from

  • Cheung, E.; D'Ari, L.; Rabinowitz, J.C.; Dyer, D.H.; Huang, J.Y.; Stoddard, B.L.
    Purification, crystallization, and preliminary X-ray studies of a bifunctional 5,10-methenyl/methylene tetrahydrofolate cyclohydrolase/dehydrogenase from Escherichia coli (1997), Proteins, 27, 322-324.
    View publication on PubMed
Search for more literature for 3.5.4.9

Cloned(Commentary)

Cloned (Comment) Organism
DNA fragment containing the bifunctional enzyme from Escherichia coli BE cloned and transformed into Escherichia coli BL21 for protein expression Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
small platelike crystals belong to body centered orthorhombic space group I222 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 2 * 32000 Drosophila melanogaster
32000
-
 2 * 32000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O Drosophila melanogaster
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Escherichia coli
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Homo sapiens
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Rattus norvegicus
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O Escherichia coli BE
-
 10-formyltetrahydrofolate
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
 bifunctional enzyme
-
Escherichia coli
-
 bifunctional enzyme
-
Escherichia coli BE
-
 bifunctional enzyme
-
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O
-
 Drosophila melanogaster 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Homo sapiens 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Rattus norvegicus 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
 ?
5,10-methenyltetrahydrofolate + H2O
-
 Escherichia coli BE 10-formyltetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae
dimer 2 * 32000 Drosophila melanogaster
dimer 2 * 32000 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Drosophila melanogaster
8.5
-
-
 Escherichia coli