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Literature summary for 3.5.4.9 extracted from
- Methylenetetrahydrofolate dehydrogenase-cyclohydrolase from Photobacterium phosphoreum shares properties with a mammalian mitochondrial homologue (1996), Biochim. Biophys. Acta, 1296, 47-54.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Homo sapiens |  |
| phosphate | - |
Photobacterium phosphoreum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amplified genomic library transformed into yest YA3-1, yeast-DNA transformed into Escherichia coli K38 pBKe, gene folD encoding PPDC expressed | Photobacterium phosphoreum |
| bifunctional dehydrogenase/cyclohydrolase portion of the cytoplasmic trifunctional enzyme cloned and heterologous expressed in the yeast strain YA3-1 | Homo sapiens |
| cDNA encoding trifunctional enzyme used to complete the purine auxotrophic yeast strain YA3-1 | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
(6R,S)-methenyltetrahydrofolate | - |
Photobacterium phosphoreum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Drosophila melanogaster | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Mus musculus | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Homo sapiens | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Rattus norvegicus | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Photobacterium phosphoreum | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Clostridium formicaceticum | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Spodoptera frugiperda | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Acetobacterium woodii | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Spodoptera frugiperda Sf9 | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacterium woodii | - |
bifunctional enzyme | - |
| Clostridium formicaceticum | - |
ATCC 23439, monofunctional enzyme | - |
| Drosophila melanogaster | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human, bifunctional mitochondrial enzyme, trifunctional cytoplasmic enzyme | - |
| Mus musculus | - |
mouse, bifunctional mitochondrial enzyme | - |
| Photobacterium phosphoreum | - |
marine bioluminescent bacterium, bifunctional enzyme | - |
| Rattus norvegicus | - |
rat, trifunctional cytoplasmic enzyme | - |
| Saccharomyces cerevisiae | - |
cytoplasmic, mitochondrial | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Spodoptera frugiperda | - |
- |
- |
| Spodoptera frugiperda Sf9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein expressed in Escherichia coli K38 | Photobacterium phosphoreum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 97.4 | - |
recombinant enzyme | Photobacterium phosphoreum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6R,S)-methenyltetrahydrofolate + H2O | - |
Homo sapiens | ? | - |
? | |
| (6R,S)-methenyltetrahydrofolate + H2O | - |
Photobacterium phosphoreum | ? | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Drosophila melanogaster | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Mus musculus | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Homo sapiens | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Rattus norvegicus | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Photobacterium phosphoreum | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Clostridium formicaceticum | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Spodoptera frugiperda | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Acetobacterium woodii | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Spodoptera frugiperda Sf9 | 10-formyltetrahydrofolate | - |
? | |
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