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Literature summary for 3.5.4.9 extracted from
- The occurrence and properties of methenyltetrahydrofolate cyclohydrolase in plants (1973), Plant Cell Physiol., 14, 319-327.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| unstable, stabilized by addition of 25% glycerol | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | slightly retards enzyme activity | Pisum sativum |  |
| methotrexate | slightly retards enzyme activity | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
5,10-methenyltetrahydrofolate | - |
Oryctolagus cuniculus | |
| 0.04 | - |
5,10-methenyltetrahydrofolate | - |
Pisum sativum | |
| 0.075 | - |
5,10-methenyltetrahydrofolate | - |
Oryctolagus cuniculus | |
| 0.2 | - |
10-formyltetrahydrofolate | - |
Pisum sativum | |
| 0.25 | - |
5,10-methenyltetrahydrofolate | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Pisum sativum | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate | Bos taurus | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | Oryctolagus cuniculus | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | Pisum sativum | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Asparagus officinalis | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Bos taurus | - |
10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Oryctolagus cuniculus | - |
10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Spinacia oleracea | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Pisum sativum | - |
10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | Lactuca sativa | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Lentinula edodes | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Flammulina velutipes | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Brassica sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Vigna mungo | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Trifolium sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Petroselinum sp. | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Allium sp. | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium sp. | - |
onion | - |
| Asparagus officinalis | - |
asparagus | - |
| Bos taurus | - |
beef | - |
| Brassica sp. | - |
cabbage | - |
| Flammulina velutipes | - |
enokitake fungus | - |
| Lactuca sativa | - |
- |
- |
| Lentinula edodes | - |
shiitake fungus | - |
| Oryctolagus cuniculus | - |
rabbit | - |
| Petroselinum sp. | - |
parsley | - |
| Pisum sativum | - |
pea, L. var. Alaska | - |
| Spinacia oleracea | - |
spinach | - |
| Trifolium sp. | - |
trefoil | - |
| Vigna mungo | - |
mappe bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | - |
Allium sp. | - |
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Pisum sativum | - |
| leaf | - |
Lactuca sativa | - |
| leaf | - |
Brassica sp. | - |
| leaf | - |
Trifolium sp. | - |
| leaf | - |
Petroselinum sp. | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
| petiole | - |
Spinacia oleracea | - |
| root | - |
Spinacia oleracea | - |
| root | - |
Pisum sativum | - |
| seedling | - |
Pisum sativum | - |
| sprout | - |
Pisum sativum | - |
| sprout | - |
Vigna mungo | - |
| sprout | green | Asparagus officinalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.28 | - |
0 day-old seedling | Pisum sativum |
| 0.37 | - |
9 day-old seedling | Pisum sativum |
| 0.44 | - |
3 day-old seedling | Pisum sativum |
| 0.5 | - |
- |
Trifolium sp. |
| 0.5 | - |
5 day-old seedling | Pisum sativum |
| 0.55 | - |
7 day-old seedling | Pisum sativum |
| 0.72 | - |
6 day-old sprout | Pisum sativum |
| 0.87 | - |
- |
Vigna mungo |
| 1.04 | - |
- |
Lentinula edodes |
| 1.04 | - |
- |
Allium sp. |
| 1.3 | - |
- |
Lactuca sativa |
| 1.35 | - |
- |
Flammulina velutipes |
| 1.9 | - |
- |
Brassica sp. |
| 2.1 | - |
- |
Petroselinum sp. |
| 2.4 | - |
source leaf | Spinacia oleracea |
| 2.7 | - |
source root | Spinacia oleracea |
| 3.7 | - |
source petiole | Spinacia oleracea |
| 4.5 | - |
- |
Asparagus officinalis |
| 32.9 | - |
purified enzyme | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 10-formyltetrahydrofolate | - |
Bos taurus | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | - |
Oryctolagus cuniculus | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 10-formyltetrahydrofolate | - |
Pisum sativum | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Asparagus officinalis | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Bos taurus | 10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Oryctolagus cuniculus | 10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Spinacia oleracea | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Pisum sativum | 10-formyltetrahydrofolate | - |
r | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Lactuca sativa | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Lentinula edodes | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Flammulina velutipes | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Brassica sp. | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Vigna mungo | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Trifolium sp. | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Petroselinum sp. | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Allium sp. | 10-formyltetrahydrofolate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | 67% activity lost at 50°C for 5 min, 80% activity lost at 50°C for 10 min, 91% activity lost at 60°C for 5 min | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | - |
- |
Pisum sativum |
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