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Literature summary for 3.5.4.9 extracted from
- Purification and properties of 5,10-methenyltetrahydrofolate cyclohydrolase from Clostridium formicoaceticum (1982), J. Biol. Chem., 257, 3833-3836.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
5,10-methenyltetrahydrofolate | pH 7.2, 35°C | Clostridium formicaceticum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
2 * 25500, SDS-PAGE | Clostridium formicaceticum |
| 41000 | - |
sedimentation equilibrium centrifugation | Clostridium formicaceticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | Gallus gallus | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Sus scrofa | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Ovis aries | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Clostridium formicaceticum | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Acetobacterium woodii | - |
10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | Gottschalkia acidurici | - |
10-formyltetrahydrofolate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacterium woodii | - |
bifunctional enzyme | - |
| Clostridium formicaceticum | - |
ATCC 23439, monofunctional enzyme | - |
| Escherichia coli | - |
- |
- |
| Gallus gallus | - |
chicken, trifunctional enzyme | - |
| Gottschalkia acidurici | - |
- |
- |
| Moorella thermoacetica | - |
- |
- |
| Ovis aries | - |
sheep, trifunctional enzyme | - |
| Saccharomyces cerevisiae | - |
yeast, trifunctional enzyme | - |
| Sus scrofa | - |
pig, trifunctional enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium formicaceticum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 469 | - |
- |
Clostridium formicaceticum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 50 mM phosphate buffer, pH 7.0, stable for at least 1 week | Clostridium formicaceticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methenyltetrahydrofolate + H2O | - |
Gallus gallus | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Sus scrofa | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Ovis aries | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Clostridium formicaceticum | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Acetobacterium woodii | 10-formyltetrahydrofolate | - |
? | |
| 5,10-methenyltetrahydrofolate + H2O | - |
Gottschalkia acidurici | 10-formyltetrahydrofolate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | - |
Saccharomyces cerevisiae |
| dimer | 2 * 25500, SDS-PAGE | Clostridium formicaceticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
loses activity rapidly above, after 2 min at 55°C 33% activity remains, after 2 min at 65°C 4% activity remains | Clostridium formicaceticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 7.6 | - |
Clostridium formicaceticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8 | - |
Clostridium formicaceticum |
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