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Literature summary for 3.5.4.5 extracted from
- Cytidine deaminase from two extremophilic bacteria: cloning, expression and comparison of their structural stability (2001), Protein Eng., 14, 807-813.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | [Bacillus] caldolyticus |
| expression in Escherichia coli | Sporosarcina psychrophila |
General Stability
| General Stability | Organism |
|---|---|
| irreversible denaturation after 30 min at 72°C | Bacillus subtilis |
| irreversible denaturation at 72°C after 30 min | Sporosarcina psychrophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14237 | - |
4 * 14237, calculation from nucleotide sequence | [Bacillus] caldolyticus |
| 14600 | - |
4 * 14600, calculation from nucleotide sequence | Sporosarcina psychrophila |
| 15000 | - |
4 * 15000, SDS-PAGE | [Bacillus] caldolyticus |
| 15000 | - |
4 * 15000, SDS-PAGE | Sporosarcina psychrophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Sporosarcina psychrophila | Q9S3M0 | - |
- |
| [Bacillus] caldolyticus | Q9R2S1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Bacillus] caldolyticus |
- |
Sporosarcina psychrophila |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| after partial denaturation at 72°C for 30 min and following renaturation on ice, the enzyme retains its enzymatic activity | [Bacillus] caldolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxycytidine + H2O | - |
Bacillus subtilis | deoxyuridine + NH3 | - |
? |  |
| deoxycytidine + H2O | - |
[Bacillus] caldolyticus | deoxyuridine + NH3 | - |
? | |
| deoxycytidine + H2O | - |
Sporosarcina psychrophila | deoxyuridine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 15000, SDS-PAGE | [Bacillus] caldolyticus |
| tetramer | 4 * 15000, SDS-PAGE | Sporosarcina psychrophila |
| tetramer | 4 * 14237, calculation from nucleotide sequence | [Bacillus] caldolyticus |
| tetramer | 4 * 14600, calculation from nucleotide sequence | Sporosarcina psychrophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDABcald | - |
[Bacillus] caldolyticus |
| CDABpsy | - |
Sporosarcina psychrophila |
| CDABsub | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Sporosarcina psychrophila |
| 37 | - |
- |
Bacillus subtilis |
| 56 | 62 | - |
[Bacillus] caldolyticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 6 | 70 | 6°C: 45% of maximal activity, 70°C: 35% of maximal activity | Sporosarcina psychrophila |
| 12 | 66 | 12°C: about 55% of maximal activity, 66°C: about 65% of maximal activity | Bacillus subtilis |
| 34 | 74 | 34°C: 45% of maximal activity, 74°C: 70% of maximal activity | [Bacillus] caldolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable up to | [Bacillus] caldolyticus |
| 72 | - |
30 min, irreversible denaturation | Bacillus subtilis |
| 72 | - |
30 min, irreversible denaturation | Sporosarcina psychrophila |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | chromatofocusing | - |
4.2 |
| Sporosarcina psychrophila | chromatofocusing | - |
4.2 |
| [Bacillus] caldolyticus | chromatofocusing | - |
4.9 |
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