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Literature summary for 3.5.4.5 extracted from

  • Cacciamani, T.; Vita, A.; Cristalli, G.; Vincenzetti, S.; Natalini, P.; Ruggieri, S.; Amici, A.; Magni, G.
    Purification of human cytidine deaminase: Molecular and enzymatic characterization and inhibition by synthetic pyrimidine analogs (1991), Arch. Biochem. Biophys., 290, 285-292.
    View publication on PubMed
Search for more literature for 3.5.4.5

Application

Application Comment Organism
medicine enzyme limits the effectiveness of the antineoplastic agent cytosine arabinoside or aracytidine in the treatment of acute myeloblastic leukemias, chemotherapeutic use of the inhibitor THU to protect antileukemic agents from deaminase attack, inhibitors of cytidine deaminase prolongs the plasma halflife of antitumor or antiviral drugs Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyuridine
-
 Homo sapiens
2-thio-6-azarudine
-
 Homo sapiens
3'-azido-3'-deoxythymidine
-
 Homo sapiens
3-deazauridine
-
 Homo sapiens
4-amino-1-(5-hydroxypentyl)pyrimidin-2(1H)-one
-
 Homo sapiens
4-amino-1-hexyl-5,6-dihydropyrimidin-2(1H)-one
-
 Homo sapiens
4-hydroxy-1-(beta-D-ribofuranosyl)piperidin-2-one
-
 Homo sapiens
5,6-Dihydrouridine
-
 Escherichia coli
5,6-Dihydrouridine
-
 Homo sapiens
5-(Chloromercuri)cytidine
-
 Escherichia coli
5-(Chloromercuri)cytidine
-
 Homo sapiens
5-Bromouridine
-
 Homo sapiens
5-fluoro-2'-deoxyuridine
-
 Homo sapiens
5-fluorouridine
-
 Homo sapiens
5-iodo-2'-deoxyuridine
-
 Homo sapiens
5-methyluridine
-
 Homo sapiens
5-{(1E)-3-[(5-carboxypentyl)amino]-3-oxoprop-1-en-1-yl}-5,6-dihydrocytidine
-
 Homo sapiens
6-azauridine
-
 Homo sapiens
Ag+
-
 Homo sapiens
ara-U
-
 Homo sapiens
CMP
-
 Homo sapiens
Cu2+
-
 Escherichia coli
Cu2+
-
 Homo sapiens
Cu2+
-
 Mus musculus
dCMP
-
 Homo sapiens
deoxyadenosine
-
 Escherichia coli
deoxyadenosine
-
 Homo sapiens
DTNB
-
 Homo sapiens
DTNB
-
 Mus musculus
dTTP
-
 Homo sapiens
dTTP
-
 Mus musculus
Hg+
-
 Escherichia coli
Hg+
-
 Homo sapiens
Hg+
-
 Mus musculus
N-ethylmaleimide weak inhibition Homo sapiens
N-ethylmaleimide
-
 Mus musculus
p-chloromercuribenzoate
-
 Homo sapiens
pseudouridine
-
 Homo sapiens
thymidine
-
 Escherichia coli
thymidine
-
 Homo sapiens
thymine riboside
-
 Escherichia coli
thymine riboside
-
 Homo sapiens
uridine
-
 Escherichia coli
uridine
-
 Homo sapiens
UTP
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13500
-
 4 * 13500, SDS-PAGE Homo sapiens
52000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytidine + H2O Gallus gallus
-
 uridine + NH3
-
 ?
cytidine + H2O Mus musculus
-
 uridine + NH3
-
 ?
cytidine + H2O Escherichia coli
-
 uridine + NH3
-
 ?
cytidine + H2O Homo sapiens
-
 uridine + NH3
-
 ?
cytidine + H2O Canis lupus familiaris
-
 uridine + NH3
-
 ?
cytidine + H2O Mus musculus BALB/c
-
 uridine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Escherichia coli
-
-
-
Gallus gallus
-
 chicken
-
Homo sapiens
-
 human
-
Mus musculus
-
 mouse
-
Mus musculus
-
 BALB/c
-
Mus musculus BALB/c
-
 BALB/c
-
no activity in Lactobacillus sp.
-
-
-
no activity in Moraxella sp.
-
 Neisseria meningitidis
-
no activity in Pseudomonas sp.
-
 P. acidovorans
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
granulocyte normal and leukemic Homo sapiens
-
liver
-
 Gallus gallus
-
liver
-
 Mus musculus
-
liver
-
 Homo sapiens
-
liver
-
 Canis lupus familiaris
-
lymphocyte
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
reticulocyte
-
 Homo sapiens
-
spleen
-
 Mus musculus
-
spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64.1
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol appears to be necessary to prevent inactivation from occuring after freezing and thawing Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine + H2O
-
 Homo sapiens ?
-
 ?
1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine + H2O
-
 Canis lupus familiaris ?
-
 ?
1-beta-D-arabinofuranosylcytosine + H2O
-
 Mus musculus ?
-
 ?
1-beta-D-arabinofuranosylcytosine + H2O
-
 Escherichia coli ?
-
 ?
1-beta-D-arabinofuranosylcytosine + H2O
-
 Homo sapiens ?
-
 ?
1-beta-D-arabinofuranosylcytosine + H2O
-
 Canis lupus familiaris ?
-
 ?
2'-deoxycytidine + H2O
-
 Mus musculus 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Escherichia coli 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Homo sapiens 2'-deoxyuridine + NH3
-
 ?
2'-deoxycytidine + H2O
-
 Mus musculus BALB/c 2'-deoxyuridine + NH3
-
 ?
2'-fluoro-2'-deoxycytidine + H2O
-
 Escherichia coli 2'-fluoro-2'-deoxyuridine + NH3
-
 ?
2'-fluoro-2'-deoxycytidine + H2O
-
 Homo sapiens 2'-fluoro-2'-deoxyuridine + NH3
-
 ?
5'-methyl-2'-deoxycytidine + H2O
-
 Mus musculus 5'-methyl-2-deoxyuridine + NH3
-
 ?
5'-methyl-2'-deoxycytidine + H2O
-
 Escherichia coli 5'-methyl-2-deoxyuridine + NH3
-
 ?
5'-methyl-2'-deoxycytidine + H2O
-
 Homo sapiens 5'-methyl-2-deoxyuridine + NH3
-
 ?
5'-methyl-2'-deoxycytidine + H2O
-
 Mus musculus BALB/c 5'-methyl-2-deoxyuridine + NH3
-
 ?
5-azacytidine + H2O
-
 Escherichia coli 5-azauridine + NH3
-
 ?
5-azacytidine + H2O
-
 Homo sapiens 5-azauridine + NH3
-
 ?
5-azadeoxycytidine + H2O
-
 Homo sapiens 5-azadeoxyuridine + NH3
-
 ?
5-iodo-2'-deoxycytidine
-
 Homo sapiens 5-iodo-2'-deoxyuridine + NH3
-
 ?
5-iododeoxycytidine + H2O
-
 Mus musculus 5-iododeoxyuridine + NH3
-
 ?
5-iododeoxycytidine + H2O
-
 Homo sapiens 5-iododeoxyuridine + NH3
-
 ?
5-methylcytidine + H2O
-
 Escherichia coli 5-methyluridine + NH3
-
 ?
5-methylcytidine + H2O
-
 Homo sapiens 5-methyluridine + NH3
-
 ?
cytidine + H2O
-
 Gallus gallus uridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus uridine + NH3
-
 ?
cytidine + H2O
-
 Escherichia coli uridine + NH3
-
 ?
cytidine + H2O
-
 Homo sapiens uridine + NH3
-
 ?
cytidine + H2O
-
 Canis lupus familiaris uridine + NH3
-
 ?
cytidine + H2O
-
 Mus musculus BALB/c uridine + NH3
-
 ?
cytosine arabinoside + H2O
-
 Mus musculus uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Escherichia coli uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Homo sapiens uridine arabinoside + NH3
-
 ?
cytosine arabinoside + H2O
-
 Mus musculus BALB/c uridine arabinoside + NH3
-
 ?
deoxycytidine + H2O
-
 Mus musculus deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Escherichia coli deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Homo sapiens deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Canis lupus familiaris deoxyuridine + NH3
-
 ?
deoxycytidine + H2O
-
 Mus musculus BALB/c deoxyuridine + NH3
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information 3-methylcytidine, cytosine and cyclocytidine are no substrates Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Gallus gallus
tetramer 4 * 13500, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
cytidine/2'-deoxycytidine aminohydrolase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10.5 deoxycytidine as substrate, beyond these values irreversible inactivation occurs Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 10.5 deoxycytidine as substrate, beyond these extremes rapid inactivation Homo sapiens