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Literature summary for 3.5.4.5 extracted from
- Purification and properties of cytidine deaminase from escherichia coli (1984), J. Biol. Chem., 259, 13615-13620.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-(Chloromercuri)cytidine | - |
Escherichia coli |  |
| 5-(Chloromercuri)cytidine | - |
Homo sapiens | |
| Borate | - |
Escherichia coli | |
| mercaptoethanol | - |
Escherichia coli | |
| Mersalyl acid | - |
Escherichia coli | |
| Mersalyl acid | - |
Mus musculus | |
| Mersalyl acid | - |
Saccharomyces cerevisiae | |
| p-mercuribenzoate | enzyme from leukemic spleen, enzyme from normal spleen is much less sensitive | Mus musculus | |
| p-mercuribenzoate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.058 | - |
2'-deoxycytidine | - |
Escherichia coli | |
| 0.07 | - |
2'-fluoro-2'-deoxy-5-iodocytosine arabinoside | - |
Escherichia coli | |
| 0.22 | - |
cytidine | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Escherichia coli |
| 51000 | - |
human granulocytes | Homo sapiens |
| 54000 | - |
gel filtration | Escherichia coli |
| 57000 | - |
- |
Saccharomyces cerevisiae |
| 74000 | - |
spleen | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytidine + H2O | Mus musculus | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Homo sapiens | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Saccharomyces cerevisiae | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Ovis aries | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Zea mays | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Escherichia coli B / ATCC 11303 | - |
uridine + NH3 | - |
? | |
| cytidine + H2O | Mus musculus BALB/c | - |
uridine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
| Homo sapiens | - |
human | - |
| Mus musculus | - |
mouse | - |
| Mus musculus | - |
BALB/c | - |
| Mus musculus BALB/c | - |
BALB/c | - |
| Ovis aries | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Zea mays | - |
corn | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
- |
Escherichia coli |
- |
Homo sapiens |
- |
Saccharomyces cerevisiae |
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Mus musculus | - |
| liver | - |
Homo sapiens | - |
| liver | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 118 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in presence of 5 mM mercaptoethanol, reasonably stable, retains 47% of its activity after 3 months, freezing inactivates the enzyme, less than 30% recovery of activity after 1 freeze-thaw cycle | Escherichia coli |
| 4°C, stable for several days, may be kept for months without significant loss of activity if frozen | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxycytidine + H2O | - |
Mus musculus | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Escherichia coli | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Homo sapiens | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Saccharomyces cerevisiae | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Escherichia coli B / ATCC 11303 | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-deoxycytidine + H2O | - |
Mus musculus BALB/c | 2'-deoxyuridine + NH3 | - |
? | |
| 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O | - |
Escherichia coli | ? | - |
? | |
| 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O | - |
Homo sapiens | ? | - |
? | |
| 2'-fluoro-2'-deoxy-5-iodocytosine arabinoside + H2O | - |
Escherichia coli B / ATCC 11303 | ? | - |
? | |
| 2'-O-methylcytidine + H2O | - |
Escherichia coli | ? | - |
? | |
| 5-azacytidine + H2O | - |
Escherichia coli | 5-azauridine + NH3 | - |
? | |
| 5-azacytidine + H2O | - |
Homo sapiens | 5-azauridine + NH3 | - |
? | |
| 5-azacytidine + H2O | - |
Escherichia coli B / ATCC 11303 | 5-azauridine + NH3 | - |
? | |
| cytidine + H2O | - |
Mus musculus | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Homo sapiens | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Saccharomyces cerevisiae | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Ovis aries | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Zea mays | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Escherichia coli B / ATCC 11303 | uridine + NH3 | - |
? | |
| cytidine + H2O | - |
Mus musculus BALB/c | uridine + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Mus musculus | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Escherichia coli | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Homo sapiens | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Escherichia coli B / ATCC 11303 | uridine arabinoside + NH3 | - |
? | |
| cytosine arabinoside + H2O | - |
Mus musculus BALB/c | uridine arabinoside + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDase | - |
Escherichia coli |
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