Application | Comment | Organism |
---|---|---|
pharmacology | Plasmodium falciparum-specific inhibitors of adenosine deaminase have potential for development as antimalarials without inhibition of host enzyme | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21 | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxycoformycin | a natural product transition state analogue inhibitor | Bos taurus | |
2'-deoxycoformycin | a natural product transition state analogue inhibitor | Homo sapiens | |
2'-deoxycoformycin | a natural product transition state analogue inhibitor | Plasmodium falciparum | |
5'-methylthio-2'-deoxycoformycin | - |
Plasmodium falciparum | |
5'-methylthiocoformycin | - |
Plasmodium falciparum | |
coformycin | a natural product transition state analogue inhibitor | Bos taurus | |
coformycin | a natural product transition state analogue inhibitor | Homo sapiens | |
coformycin | a natural product transition state analogue inhibitor | Plasmodium falciparum | |
additional information | no inhibition of the bovine enzyme by 5'-methylthiocoformycin and 5'-methylthio-2'-deoxycoformycin | Bos taurus | |
additional information | no inhibition of the human enzyme by 5'-methylthiocoformycin and 5'-methylthio-2'-deoxycoformycin | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | Plasmodium falciparum | - |
5'-methylthioinosine + NH3 | - |
? | |
adenosine + H2O | Homo sapiens | - |
inosine + NH3 | - |
? | |
adenosine + H2O | Bos taurus | - |
inosine + NH3 | - |
? | |
adenosine + H2O | Plasmodium falciparum | adenosine deamination is involved in the essential pathway of purine salvage in Plasmodium falciparum, and inhibition of purine salvage results in parasite death | inosine + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Homo sapiens | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Homo sapiens | - |
commercial preparation | - |
Bos taurus | - |
erythrocyte | - |
Homo sapiens | - |
spleen | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + H2O | - |
Plasmodium falciparum | 5'-methylthioinosine + NH3 | - |
? | |
adenosine + H2O | - |
Homo sapiens | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Bos taurus | inosine + NH3 | - |
? | |
adenosine + H2O | - |
Plasmodium falciparum | inosine + NH3 | - |
? | |
adenosine + H2O | adenosine deamination is involved in the essential pathway of purine salvage in Plasmodium falciparum, and inhibition of purine salvage results in parasite death | Plasmodium falciparum | inosine + NH3 | - |
? | |
additional information | no activity with 5'-methylthioadenosine | Homo sapiens | ? | - |
? | |
additional information | no activity with 5'-methylthioadenosine | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADA | - |
Homo sapiens |
ADA | - |
Bos taurus |
ADA | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Bos taurus |
30 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
7 | - |
assay at | Bos taurus |
7 | - |
assay at | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000043 | - |
5'-methylthiocoformycin | - |
Plasmodium falciparum | |
0.00000079 | - |
5'-methylthio-2'-deoxycoformycin | - |
Plasmodium falciparum |