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Literature summary for 3.5.4.4 extracted from

  • Pickard, M.A.
    Purification and some properties of the soluble and membrane-bound adenosine deaminases of Micrococcus sodonensis ATCC 11880 and their distribution within the family Micrococcaceae (1975), Can. J. Biochem., 53, 344-353.
    View publication on PubMed
Search for more literature for 3.5.4.4

General Stability

General Stability Organism
Mg2+ or Mn2+ required during purification procedure, addition of serum albumin protects against loss of activity by freezing Micrococcus sodonensis

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Micrococcus sodonensis
aminoxyacetic acid
-
 Micrococcus sodonensis
EDTA
-
 Micrococcus sodonensis
Hg2+
-
 Micrococcus sodonensis
iodoacetamide
-
 Micrococcus sodonensis
iodoacetic acid
-
 Micrococcus sodonensis
N-ethylmaleimide
-
 Micrococcus sodonensis
p-chloromercuribenzoate
-
 Micrococcus sodonensis
Pb2+
-
 Micrococcus sodonensis
Zn2+
-
 Micrococcus sodonensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Micrococcus sodonensis 5737
-
integral to plasma membrane
-
 Micrococcus sodonensis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 gel filtration Micrococcus sodonensis

Organism

Organism UniProt Comment Textmining
Kocuria rosea
-
 NCIB 8175, soluble enzyme, membrane-bound enzyme
-
Macrococcus caseolyticus
-
 NCIB 3251, soluble enzyme, membrane-bound enzyme
-
Micrococcus candidus
-
 NCIB 8610, soluble enzyme, membrane-bound enzyme
-
Micrococcus freudenreichii
-
 ATCC 407, soluble enzyme, membrane-bound enzyme
-
Micrococcus luteus
-
 ATCC 9341, soluble enzyme, membrane-bound enzyme
-
Micrococcus luteus
-
 NCIB 9278, soluble enzyme, membrane-bound enzyme
-
Micrococcus sodonensis
-
 ATCC 11880, soluble enzyme, membrane-bound enzyme
-
Micrococcus sp.
-
 NC1B 8165, soluble enzyme, membrane-bound enzyme
-
Staphylococcus aerogenes
-
 soluble enzyme, membrane-bound enzyme
-
Staphylococcus aureus
-
 soluble enzyme, membrane-bound enzyme
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus sodonensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 soluble enzyme Micrococcus sp.
0.076
-
 membrane-bound enzyme Macrococcus caseolyticus
0.136
-
 membrane-bound enzyme Micrococcus sp.
0.16
-
 soluble enzyme Macrococcus caseolyticus
0.164
-
 membrane-bound enzyme Micrococcus luteus
0.191
-
 membrane-bound enzyme Staphylococcus aerogenes
0.281
-
 membrane-bound enzyme Micrococcus candidus
0.366
-
 membrane-bound enzyme Kocuria rosea
0.401
-
 membrane-bound enzyme Staphylococcus aureus
0.622
-
 membrane-bound enzyme Micrococcus luteus
1.28
-
 membrane-bound enzyme Micrococcus freudenreichii
3.04
-
 soluble enzyme Kocuria rosea
6.07
-
 soluble enzyme Micrococcus luteus
9.07
-
 soluble enzyme Micrococcus luteus
10.5
-
 soluble enzyme Micrococcus candidus
19.1
-
 soluble enzyme Micrococcus freudenreichii
372
-
 membrane-bound enzyme Micrococcus sodonensis
2180
-
 soluble enzyme Micrococcus sodonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-isopropylideneadenosine + H2O
-
 Micrococcus sodonensis 2',3'-isopropylideneinosine + NH3
-
 ?
2'-deoxyadenosine + H2O
-
 Micrococcus sodonensis 2'-deoxyinosine + NH3
-
 ?
2-amino-6-chloropurine riboside + H2O
-
 Micrococcus sodonensis guanosine + Cl-
-
 ?
3'-deoxyadenosine + H2O
-
 Micrococcus sodonensis 3'-deoxyinosine + NH3
-
 ?
6-chloropurine riboside + H2O
-
 Micrococcus sodonensis inosine + Cl-
-
 ?
adenosine + H2O
-
 Micrococcus sodonensis inosine + NH3
-
 ?