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Literature summary for 3.5.4.3 extracted from

  • Bitra, A.; Hussain, B.; Tanwar, A.S.; Anand, R.
    Identification of function and mechanistic insights of guanine deaminase from Nitrosomonas europaea: role of the C-terminal loop in catalysis (2013), Biochemistry, 52, 3512-3522.
    View publication on PubMed
Search for more literature for 3.5.4.3

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with 8-azaguanine, hanging drop vapor diffusion method Nitrosomonas europaea

Protein Variants

Protein Variants Comment Organism
E143X the mutation results in a complete loss of activity Nitrosomonas europaea
E79X the mutation results in a complete loss of activity Nitrosomonas europaea
additional information the mutant form of the enzyme with the deletion of the extreme nine amino acids does not exhibit any enzyme activity Nitrosomonas europaea

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ammeline + H2O Nitrosomonas europaea
-
 ?
-
 ?
guanine + H2O Nitrosomonas europaea
-
 xanthine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Nitrosomonas europaea Q82Y41
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ammeline + H2O
-
 Nitrosomonas europaea ?
-
 ?
guanine + H2O
-
 Nitrosomonas europaea xanthine + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Nitrosomonas europaea

Synonyms

Synonyms Comment Organism
NE0047
-
 Nitrosomonas europaea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
120
-
 guanine pH and temperature not specified in the publication Nitrosomonas europaea