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Literature summary for 3.5.4.3 extracted from

  • Kimm, S.W.; Park, J.B.; Lee, I.S.
    Purification and characterization of guanine aminohydrolase from human liver (1985), Korean J. Biochem., 17, 139-148.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
5-aminoimidazole-4-carboxamide 29% inhibition Homo sapiens
5-aminoimidazole-4-carboxamide
-
Oryctolagus cuniculus
5-aminoimidazole-4-carboxamide
-
Rattus norvegicus
adenine
-
Homo sapiens
adenosine
-
Homo sapiens
hypoxanthine slight inhibition of 8% Homo sapiens
Inosine slight inhibition of 5% Homo sapiens
p-hydroxymercuribenzoate noncompetitive inhibitor, 0.1 mM, 37°C, rapid inactivation Homo sapiens
p-hydroxymercuribenzoate
-
Oryctolagus cuniculus
p-hydroxymercuribenzoate
-
Rattus norvegicus
xanthine
-
Homo sapiens
Xanthosine
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
guanine at pH 8 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
-
Homo sapiens
55000
-
liver, single polypeptide chain, gel filtration Rattus norvegicus
55000
-
liver, single polypeptide chain, gel filtration Oryctolagus cuniculus
100000
-
-
Oryctolagus cuniculus
100000
-
gel filtration Homo sapiens
110000
-
-
Rattus norvegicus
110000
-
liver, gel filtration Homo sapiens
110000
-
liver, gel filtration Oryctolagus cuniculus
220000
-
gel filtration without mercaptoethanol Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + H2O Homo sapiens
-
xanthine + NH3
-
ir
guanine + H2O Rattus norvegicus
-
xanthine + NH3
-
ir
guanine + H2O Oryctolagus cuniculus
-
xanthine + NH3
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
human
-
Oryctolagus cuniculus
-
rabbit
-
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.61
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-azaguanine + H2O
-
Rattus norvegicus 8-azaxanthine + NH3
-
?
8-azaguanine + H2O
-
Oryctolagus cuniculus 8-azaxanthine + NH3
-
?
8-azaguanine + H2O high substrate specificity, only 60% of the reaction rate with the natural substrate guanine Homo sapiens 8-azaxanthine + NH3
-
?
adenine + H2O
-
Homo sapiens allopurinol + NH3
-
?
adenosine + H2O
-
Homo sapiens inosine + NH3
-
?
AMP + H2O
-
Homo sapiens IMP + NH3
-
?
GMP + H2O
-
Homo sapiens XMP + NH3
-
?
guanine + H2O
-
Homo sapiens xanthine + NH3
-
ir
guanine + H2O
-
Rattus norvegicus xanthine + NH3
-
ir
guanine + H2O
-
Oryctolagus cuniculus xanthine + NH3
-
ir
guanosine + H2O
-
Homo sapiens xanthosine + NH3
-
?

Subunits

Subunits Comment Organism
dimer
-
Homo sapiens
dimer
-
Oryctolagus cuniculus
dimer 2 * 55000, 2 or 3 isoenzymes, polymeric nature, enzyme exists as monomer, dimer or tetramer Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
50% activity lost during 30 min incubation, after 60 min 40% activity remains Homo sapiens
60
-
30 min incubation, liver enzyme completely inactivated Rattus norvegicus
60
-
30 min incubation, liver enzyme completely inactivated Oryctolagus cuniculus
65
-
activity completely lost after 30 min incubation Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
Oryctolagus cuniculus
7.5 9.5
-
Rattus norvegicus
7.5 9.5
-
Oryctolagus cuniculus
7.5 9.5 beyond pH 9.5 activity decreases abruptly Homo sapiens
8
-
-
Homo sapiens
8
-
-
Rattus norvegicus
8
-
-
Oryctolagus cuniculus
8.5
-
-
Rattus norvegicus
8.5
-
-
Oryctolagus cuniculus
8.6
-
-
Oryctolagus cuniculus
9
-
-
Homo sapiens