Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoimidazole-4-carboxamide | 29% inhibition | Homo sapiens | |
5-aminoimidazole-4-carboxamide | - |
Oryctolagus cuniculus | |
5-aminoimidazole-4-carboxamide | - |
Rattus norvegicus | |
adenine | - |
Homo sapiens | |
adenosine | - |
Homo sapiens | |
hypoxanthine | slight inhibition of 8% | Homo sapiens | |
Inosine | slight inhibition of 5% | Homo sapiens | |
p-hydroxymercuribenzoate | noncompetitive inhibitor, 0.1 mM, 37°C, rapid inactivation | Homo sapiens | |
p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
xanthine | - |
Homo sapiens | |
Xanthosine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0074 | - |
guanine | at pH 8 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
- |
Homo sapiens |
55000 | - |
liver, single polypeptide chain, gel filtration | Rattus norvegicus |
55000 | - |
liver, single polypeptide chain, gel filtration | Oryctolagus cuniculus |
100000 | - |
- |
Oryctolagus cuniculus |
100000 | - |
gel filtration | Homo sapiens |
110000 | - |
- |
Rattus norvegicus |
110000 | - |
liver, gel filtration | Homo sapiens |
110000 | - |
liver, gel filtration | Oryctolagus cuniculus |
220000 | - |
gel filtration without mercaptoethanol | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + H2O | Homo sapiens | - |
xanthine + NH3 | - |
ir | |
guanine + H2O | Rattus norvegicus | - |
xanthine + NH3 | - |
ir | |
guanine + H2O | Oryctolagus cuniculus | - |
xanthine + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Oryctolagus cuniculus | - |
rabbit | - |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.61 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-azaguanine + H2O | - |
Rattus norvegicus | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | - |
Oryctolagus cuniculus | 8-azaxanthine + NH3 | - |
? | |
8-azaguanine + H2O | high substrate specificity, only 60% of the reaction rate with the natural substrate guanine | Homo sapiens | 8-azaxanthine + NH3 | - |
? | |
adenine + H2O | - |
Homo sapiens | allopurinol + NH3 | - |
? | |
adenosine + H2O | - |
Homo sapiens | inosine + NH3 | - |
? | |
AMP + H2O | - |
Homo sapiens | IMP + NH3 | - |
? | |
GMP + H2O | - |
Homo sapiens | XMP + NH3 | - |
? | |
guanine + H2O | - |
Homo sapiens | xanthine + NH3 | - |
ir | |
guanine + H2O | - |
Rattus norvegicus | xanthine + NH3 | - |
ir | |
guanine + H2O | - |
Oryctolagus cuniculus | xanthine + NH3 | - |
ir | |
guanosine + H2O | - |
Homo sapiens | xanthosine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | - |
Oryctolagus cuniculus |
dimer | 2 * 55000, 2 or 3 isoenzymes, polymeric nature, enzyme exists as monomer, dimer or tetramer | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50% activity lost during 30 min incubation, after 60 min 40% activity remains | Homo sapiens |
60 | - |
30 min incubation, liver enzyme completely inactivated | Rattus norvegicus |
60 | - |
30 min incubation, liver enzyme completely inactivated | Oryctolagus cuniculus |
65 | - |
activity completely lost after 30 min incubation | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Oryctolagus cuniculus |
7.5 | 9.5 | - |
Rattus norvegicus |
7.5 | 9.5 | - |
Oryctolagus cuniculus |
7.5 | 9.5 | beyond pH 9.5 activity decreases abruptly | Homo sapiens |
8 | - |
- |
Homo sapiens |
8 | - |
- |
Rattus norvegicus |
8 | - |
- |
Oryctolagus cuniculus |
8.5 | - |
- |
Rattus norvegicus |
8.5 | - |
- |
Oryctolagus cuniculus |
8.6 | - |
- |
Oryctolagus cuniculus |
9 | - |
- |
Homo sapiens |