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Literature summary for 3.5.4.2 extracted from

  • Goble, A.; Zhang, Z.; Sauder, J.; Burley, S.; Swaminathan, S.; Raushel, F.
    Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine (2011), Biochemistry, 50, 6589-6597.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray structures of Pa0148 (Pseudomonas aeruginos) and AAur1117 (Arthrobacter aurescens) are determined and reveal nearly identical distorted (beta/alpha)8 barrels with a single zinc ion that is characteristic of members of the amidohydrolase superfamily Pseudomonas aeruginosa
X-ray structures of Pa0148 (Pseudomonas aeruginos) and AAur1117 (Arthrobacter aurescens) are determined and reveal nearly identical distorted (beta/alpha)8 barrels with a single zinc ion that is characteristic of members of the amidohydrolase superfamily Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
adenine pH and temperature not specified in the publication Pseudomonas aeruginosa
0.026
-
6-Chloropurine pH and temperature not specified in the publication Burkholderia sp.
0.056
-
6-Chloropurine pH and temperature not specified in the publication Alteromonas macleodii
0.07
-
2,6-diaminopurine pH and temperature not specified in the publication Alteromonas macleodii
0.088
-
N-6-methyladenine pH and temperature not specified in the publication Pseudomonas aeruginosa
0.09
-
N-6-methyladenine pH and temperature not specified in the publication Alteromonas macleodii
0.13
-
6-methoxypurine pH and temperature not specified in the publication Alteromonas macleodii
0.14
-
2,6-diaminopurine pH and temperature not specified in the publication Pseudomonas aeruginosa
0.14
-
6-methoxypurine pH and temperature not specified in the publication Burkholderia sp.
0.16
-
6-methoxypurine pH and temperature not specified in the publication Paenarthrobacter aurescens
0.17
-
adenine pH and temperature not specified in the publication Burkholderia sp.
0.2
-
adenine pH and temperature not specified in the publication Paenarthrobacter aurescens
0.2
-
adenine pH and temperature not specified in the publication Alteromonas macleodii
0.26
-
6-Chloropurine pH and temperature not specified in the publication Pseudomonas aeruginosa
0.27
-
6-methoxypurine pH and temperature not specified in the publication Pseudomonas aeruginosa
0.29
-
2,6-diaminopurine pH and temperature not specified in the publication Paenarthrobacter aurescens

Organism

Organism UniProt Comment Textmining
Alteromonas macleodii
-
-
-
Burkholderia sp.
-
-
-
Paenarthrobacter aurescens
-
-
-
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-diaminopurine + H2O
-
Pseudomonas aeruginosa ?
-
?
2,6-diaminopurine + H2O
-
Paenarthrobacter aurescens ?
-
?
2,6-diaminopurine + H2O
-
Burkholderia sp. ?
-
?
2,6-diaminopurine + H2O
-
Alteromonas macleodii ?
-
?
6-chloropurine + H2O
-
Pseudomonas aeruginosa hypoxanthine + HCl
-
?
6-chloropurine + H2O
-
Paenarthrobacter aurescens hypoxanthine + HCl
-
?
6-chloropurine + H2O
-
Burkholderia sp. hypoxanthine + HCl
-
?
6-chloropurine + H2O
-
Alteromonas macleodii hypoxanthine + HCl
-
?
6-methoxypurine + H2O
-
Pseudomonas aeruginosa ?
-
?
6-methoxypurine + H2O
-
Paenarthrobacter aurescens ?
-
?
6-methoxypurine + H2O
-
Burkholderia sp. ?
-
?
6-methoxypurine + H2O
-
Alteromonas macleodii ?
-
?
adenine + H2O
-
Pseudomonas aeruginosa hypoxanthine + NH3
-
?
adenine + H2O
-
Paenarthrobacter aurescens hypoxanthine + NH3
-
?
adenine + H2O
-
Burkholderia sp. hypoxanthine + NH3
-
?
adenine + H2O
-
Alteromonas macleodii hypoxanthine + NH3
-
?
N-6-methyladenine + H2O
-
Pseudomonas aeruginosa ?
-
?
N-6-methyladenine + H2O
-
Paenarthrobacter aurescens ?
-
?
N-6-methyladenine + H2O
-
Burkholderia sp. ?
-
?
N-6-methyladenine + H2O
-
Alteromonas macleodii ?
-
?

Synonyms

Synonyms Comment Organism
AAur1117
-
Paenarthrobacter aurescens
Pa0148
-
Pseudomonas aeruginosa
Sgx9403e
-
Alteromonas macleodii
Sgx9403g
-
Burkholderia sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
6-Chloropurine pH and temperature not specified in the publication Burkholderia sp.
0.48
-
N-6-methyladenine pH and temperature not specified in the publication Alteromonas macleodii
0.52
-
6-methoxypurine pH and temperature not specified in the publication Alteromonas macleodii
0.84
-
6-Chloropurine pH and temperature not specified in the publication Alteromonas macleodii
1.28
-
N-6-methyladenine pH and temperature not specified in the publication Pseudomonas aeruginosa
1.8
-
2,6-diaminopurine pH and temperature not specified in the publication Alteromonas macleodii
1.9
-
6-Chloropurine pH and temperature not specified in the publication Pseudomonas aeruginosa
1.9
-
6-methoxypurine pH and temperature not specified in the publication Paenarthrobacter aurescens
2.2
-
6-methoxypurine pH and temperature not specified in the publication Burkholderia sp.
3 6 adenine pH and temperature not specified in the publication Pseudomonas aeruginosa
6.9
-
2,6-diaminopurine pH and temperature not specified in the publication Paenarthrobacter aurescens
7.1
-
6-methoxypurine pH and temperature not specified in the publication Pseudomonas aeruginosa
17
-
2,6-diaminopurine pH and temperature not specified in the publication Pseudomonas aeruginosa
56
-
adenine pH and temperature not specified in the publication Burkholderia sp.
61
-
adenine pH and temperature not specified in the publication Paenarthrobacter aurescens
62
-
adenine pH and temperature not specified in the publication Alteromonas macleodii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
N-6-methyladenine pH and temperature not specified in the publication Paenarthrobacter aurescens
1.6
-
N-6-methyladenine pH and temperature not specified in the publication Burkholderia sp.
4
-
6-methoxypurine pH and temperature not specified in the publication Alteromonas macleodii
5.3
-
N-6-methyladenine pH and temperature not specified in the publication Alteromonas macleodii
7.3
-
6-Chloropurine pH and temperature not specified in the publication Pseudomonas aeruginosa
9.6
-
6-Chloropurine pH and temperature not specified in the publication Paenarthrobacter aurescens
12
-
6-methoxypurine pH and temperature not specified in the publication Paenarthrobacter aurescens
15
-
6-Chloropurine pH and temperature not specified in the publication Alteromonas macleodii
15
-
N-6-methyladenine pH and temperature not specified in the publication Pseudomonas aeruginosa
16
-
6-Chloropurine pH and temperature not specified in the publication Burkholderia sp.
17
-
6-methoxypurine pH and temperature not specified in the publication Burkholderia sp.
19
-
2,6-diaminopurine pH and temperature not specified in the publication Burkholderia sp.
24
-
2,6-diaminopurine pH and temperature not specified in the publication Paenarthrobacter aurescens
26
-
6-methoxypurine pH and temperature not specified in the publication Pseudomonas aeruginosa
28
-
2,6-diaminopurine pH and temperature not specified in the publication Alteromonas macleodii
120
-
2,6-diaminopurine pH and temperature not specified in the publication Pseudomonas aeruginosa
310
-
adenine pH and temperature not specified in the publication Paenarthrobacter aurescens
330
-
adenine pH and temperature not specified in the publication Burkholderia sp.
1400
-
adenine pH and temperature not specified in the publication Pseudomonas aeruginosa
3100
-
adenine pH and temperature not specified in the publication Alteromonas macleodii