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Literature summary for 3.5.4.13 extracted from
- A bifunctional dCTP deaminase-dUTP nucleotidohydrolase from the hyperthermophilic archaeon Methanocaldococcus jannaschii (2003), J. Biol. Chem., 278, 20667-20672.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dTTP | inhibition of deamination reaction | Methanocaldococcus jannaschii |  |
| dUTP | inhibition of deamination reaction | Methanocaldococcus jannaschii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | for dUTP, Km-value in millimolar range | Methanocaldococcus jannaschii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely required | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23300 | - |
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
| 23400 | - |
x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Methanocaldococcus jannaschii | bifunctionality of enzyme avoids release of toxic UTP within cells | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
bifunctional enzyme dCTP deaminase-dUTP nucleotidohydrolase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dCTP + H2O | - |
Methanocaldococcus jannaschii | dUTP + NH3 | - |
? | |
| ddCTP + H2O | deamination at the same rate as dCTP | Methanocaldococcus jannaschii | ddUTP + NH3 | - |
? | |
| dUTP + 2 H2O | - |
Methanocaldococcus jannaschii | dUMP + 2 phosphate | - |
? | |
| additional information | bifunctionality of enzyme avoids release of toxic UTP within cells | Methanocaldococcus jannaschii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23400, deduced from gene sequence, x * 23300, mass spectrometry | Methanocaldococcus jannaschii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
dTTP | pH 6.8, 25°C | Methanocaldococcus jannaschii | |
| 0.5 | - |
dUTP | - |
Methanocaldococcus jannaschii | |
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