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Literature summary for 3.5.4.1 extracted from

  • Zamboni, S.; Mallano, A.; Flego, M.; Ascione, A.; Dupuis, M.L.; Gellini, M.; Barca, S.; Cianfriglia, M.
    Genetic construction, expression, and characterization of a single chain anti-CEA antibody fused to cytosine deaminase from yeast (2008), Int. J. Oncol., 32, 1245-1251.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein in Escherichia coli as soluble protein Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information construction of a His6-tagged fusion protein using the yeast cytosine deaminase and a single chain fragment variable human antibody specific for carcinoembryonic antigen, CEA, cell surfaces under control of the lac promoter, the chimeric enzyme shows high affinity for binding to Mel P5 and LoVo melanoma cells and improves tumor-selective prodrug activation, overview Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein from Escherichia coli by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O activation of the prodrug to the cytotoxic drug in cancer therapy by recombinant enzyme in melanoma cells, leads to increased 5-fluorouracil-mediated cell killing, overview Saccharomyces cerevisiae 5-fluorouracil + NH3
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?

Subunits

Subunits Comment Organism
? x * 18000-20000, cytosine deaminase, SDS-PAGE, x * 45000-50000, recombinant His6-tagged yeast cytosine deaminase/single chain fragment variable human antibody fusion protein, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
in vivo assay at Saccharomyces cerevisiae