Application | Comment | Organism |
---|---|---|
medicine | cytosine deaminase gene therapy combined with radiation treatment in breast carcinoma bone tumor, which is painful and causes osteolysis, is evaluated in a 4T1murine breast carcinoma model, overview | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of the fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene in murine 4T1 breast carcinoma cells as cytosolic protein, which renders the cells highly sensitive to treatment by 5-fluorocytosine prodrug | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion gene encoding the extracellular and transmembrane domains of the human nerve growth factor receptor and the cytoplasmic portion of the yeast CD gene | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Saccharomyces cerevisiae | activation of the prodrug by the recombinant fusion enzyme, overview | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
additional information | Saccharomyces cerevisiae | cytosine deaminase gene therapy combined with radiation treatment in breast cancer is evaluated in a 4T1murine breast carcinoma model, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | activation of the prodrug by the recombinant fusion enzyme, overview | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
5-fluorocytosine + H2O | activation of the prodrug | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
additional information | cytosine deaminase gene therapy combined with radiation treatment in breast cancer is evaluated in a 4T1murine breast carcinoma model, overview | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Saccharomyces cerevisiae |