Crystallization (Comment) | Organism |
---|---|
wild-type enzyme and mutants D314G, D314A, D314S, free and in complex with mechanism-based inhibitor 5-fluoro-4-(S)-hydroxyl-3,4-dihydropyrimidine | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D314A | impaired catalytic efficiency for cytosine, improved catalytic efficiency for 5-fluorocytosine | Escherichia coli |
D314G | impaired catalytic efficiency | Escherichia coli |
D314S | impaired catalytic efficiency | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-4-(S)-hydroxyl-3,4-dihydropyrimidine | mechanism-based inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
cytosine | wild-type, pH 7.5, 37°C | Escherichia coli | |
0.3 | - |
cytosine | mutant D314G, pH 7.5, 37°C | Escherichia coli | |
1 | - |
cytosine | mutant D314S, pH 7.5, 37°C | Escherichia coli | |
2.2 | - |
cytosine | mutant D314A, pH 7.5, 37°C | Escherichia coli | |
2.8 | - |
5-fluorocytosine | mutant D314A, pH 7.5, 37°C | Escherichia coli | |
3.3 | - |
5-fluorocytosine | wild-type, pH 7.5, 37°C | Escherichia coli | |
6.6 | - |
5-fluorocytosine | mutant D314S, pH 7.5, 37°C | Escherichia coli | |
10.9 | - |
5-fluorocytosine | mutant D314G, pH 7.5, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.8 | - |
cytosine | mutant D314G, pH 7.5, 37°C | Escherichia coli | |
75.6 | - |
5-fluorocytosine | wild-type, pH 7.5, 37°C | Escherichia coli | |
97.7 | - |
5-fluorocytosine | mutant D314G, pH 7.5, 37°C | Escherichia coli | |
104 | - |
cytosine | mutant D314A, pH 7.5, 37°C | Escherichia coli | |
137 | - |
5-fluorocytosine | mutant D314A, pH 7.5, 37°C | Escherichia coli | |
165 | - |
cytosine | wild-type, pH 7.5, 37°C | Escherichia coli | |
233 | - |
5-fluorocytosine | mutant D314S, pH 7.5, 37°C | Escherichia coli | |
279 | - |
cytosine | mutant D314S, pH 7.5, 37°C | Escherichia coli |