Crystallization (Comment) | Organism |
---|---|
molecular dynamics simulation of free enzyme and in complex with cytosine, uracil and reaction intermediates | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | ligands are two cysteines, a histidine, and one water molecule | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cytosine + H2O = uracil + NH3 | E64 side-chain carboxyl group acts as proton shuttle between the Zn-bound water molecule and cytosine. In course of reaction, cytosine reorients to favor nucleophilic attack by a Zn-bound hydroxide. F114 may be important for reactant binding and product release | Saccharomyces cerevisiae |