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Literature summary for 3.5.4.1 extracted from

  • Yao, L.; Li, Y.; Wu, Y.; Liu, A.; Yan, H.
    Product release is rate-limiting in the activation of the prodrug 5-fluorocytosine by yeast cytosine deaminase (2005), Biochemistry, 44, 5940-5947.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 5-fluorocytosine pH 7.5, 25°C, transient kinetics Saccharomyces cerevisiae
0.16
-
 5-fluorocytosine pH 7.5, 25°C, steady-state kinetics Saccharomyces cerevisiae
1.1
-
 cytosine pH 7.5, 25°C, steady-state kinetics Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 pH-indicator assay for the measurement of the steady-state kinetic parameters of deamination reaction Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O release of 5-fluorouracil is rate-limiting and may involve multiple steps Saccharomyces cerevisiae 5-fluorouracil + NH3
-
 ?
cytosine + H2O
-
 Saccharomyces cerevisiae uracil + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
 5-fluorocytosine pH 7.5, 25°C, steady-state kinetics Saccharomyces cerevisiae
21
-
 5-fluorocytosine pH 7.5, 25°C, transient kinetics Saccharomyces cerevisiae
91
-
 cytosine pH 7.5, 25°C, steady-state kinetics Saccharomyces cerevisiae