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Literature summary for 3.5.4.1 extracted from
- Kinetic properties of cytosine deaminase from Serratia marcescens (1976), Agric. Biol. Chem., 40, 543-549.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pseudomonas chlororaphis subsp. aureofaciens |  |
| ATP | 10.0 mM, relative activity 154% | Serratia marcescens | |
| dAMP | 10.0 mM, relative activity 154% | Serratia marcescens | |
| GDP | 10.0 mM, relative activity 176% | Serratia marcescens | |
| GTP | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| GTP | 10.0 mM, relative activity 171% | Serratia marcescens | |
| IDP | 10.0 mM, relative activity 152% | Serratia marcescens | |
| ITP | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| ITP | 10.0 mM, relative activity 155% | Serratia marcescens | |
| L-amino acids | - |
Escherichia coli | |
| L-amino acids | - |
Serratia marcescens | |
| L-amino acids | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-asparagine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-asparagine | 10.0 mM, relative activity 150% | Serratia marcescens | |
| L-aspartic acid | - |
Escherichia coli | |
| L-aspartic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-aspartic acid | 10.0 mM, relative activity 144% | Serratia marcescens | |
| L-cysteine | 10.0 mM, relative activity 160% | Serratia marcescens | |
| L-Glutamic acid | - |
Escherichia coli | |
| L-Glutamic acid | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-Glutamic acid | 10.0 mM, relative activity 138% | Serratia marcescens | |
| L-glutamine | - |
Escherichia coli | |
| L-glutamine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-glutamine | 10.0 mM, relative activity 143% | Serratia marcescens | |
| L-histidine | - |
Escherichia coli | |
| L-histidine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-histidine | 10.0 mM, relative activity 160% | Serratia marcescens | |
| L-hydroxyproline | - |
Escherichia coli | |
| L-hydroxyproline | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-hydroxyproline | 10.0 mM, relative activity 150% | Serratia marcescens | |
| L-methionine | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| L-methionine | 10.0 mM, relative activity 150% | Serratia marcescens | |
| phosphate | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| phosphate | 1.0 mM, relative activity 170% | Serratia marcescens | |
| sulfate | 1.0 mM, relative activity 110% | Serratia marcescens | |
| sulfite | 1.0 mM, relative activity 105% | Serratia marcescens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Serratia marcescens | |
| Co2+ | - |
Serratia marcescens | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Serratia marcescens | |
| deoxyguanosine | - |
Serratia marcescens | |
| dGMP | - |
Serratia marcescens | |
| Hg2+ | - |
Escherichia coli | |
| Hg2+ | - |
Serratia marcescens | |
| Ni2+ | - |
Serratia marcescens | |
| Zn2+ | - |
Escherichia coli | |
| Zn2+ | - |
Serratia marcescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.4 | - |
cytosine | - |
Serratia marcescens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| BaCl2 | 1.0 mM, relative activity 110% | Serratia marcescens | |
| FeCl3 | - |
Pseudomonas chlororaphis subsp. aureofaciens | |
| FeCl3 | 1.0 mM, relative activity 115% | Serratia marcescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Pseudomonas chlororaphis subsp. aureofaciens | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pseudomonas chlororaphis subsp. aureofaciens | - |
- |
- |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Saccharomyces cerevisiae | - |
type Y | - |
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | thymine + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Pseudomonas chlororaphis subsp. aureofaciens | uracil + NH3 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytosine deaminase Y | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Serratia marcescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | - |
Serratia marcescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
| 9.5 | - |
with cytosine as substrate | Serratia marcescens |
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