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Literature summary for 3.5.3.12 extracted from
- Purification and characterization of putrescine synthase from cucumber seedlings. A multifunctional enzyme involved in putrescine biosynthesis (1986), J. Biosci., 10, 373-391.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| highly unstable | Cucumis sativus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE | Cucumis sativus |
| 44000 | - |
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE | Cucumis sativus |
| 48000 | - |
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE | Cucumis sativus |
| 66000 | - |
1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE | Cucumis sativus |
| 150000 | - |
gel filtration | Cucumis sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | Lathyrus sativus | - |
N-carbamoylputrescine + NH3 | - |
? |  |
| agmatine + H2O | Cucumis sativus | - |
N-carbamoylputrescine + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
cucumber | - |
| Lathyrus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lathyrus sativus |
- |
Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Cucumis sativus | - |
| seedling | - |
Lathyrus sativus | - |
| seedling | - |
Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.64 | - |
- |
Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + ADP + phosphate | - |
Cucumis sativus | ATP + putrescine + NH3 + CO2 | - |
? | |
| agmatine + H2O | - |
Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | - |
Cucumis sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase | Lathyrus sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + H2O | multifunctional enzyme, acts also as putrescine transcarbamylase, putrescine synthase, ornithine transcarbamylase and carbamate kinase | Cucumis sativus | N-carbamoylputrescine + NH3 | - |
? | |
| agmatine + ornithine + H2O + phosphate | - |
Lathyrus sativus | putrescine + citrulline + NH3 + phosphate | - |
? | |
| agmatine + ornithine + H2O + phosphate | - |
Cucumis sativus | putrescine + citrulline + NH3 + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | 1 * 66000 + 1 * 48000 + 1 * 44000 + 1 * 15000, SDS-PAGE | Cucumis sativus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| putrescine synthase | - |
Lathyrus sativus |
| putrescine synthase | - |
Cucumis sativus |
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