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Literature summary for 3.5.2.6 extracted from

  • Aitha, M.; Marts, A.R.; Bergstrom, A.; Moeller, A.J.; Moritz, L.; Turner, L.; Nix, J.C.; Bonomo, R.A.; Page, R.C.; Tierney, D.L.; Crowder, M.W.
    Biochemical, mechanistic, and spectroscopic characterization of metallo-beta-lactamase VIM-2 (2014), Biochemistry, 53, 7321-7331.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
gene blaVIM-2, recombinant Co2+-enzyme expression in Escherichia coli strain BL21(DE3) in in minimal medium Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, sitting drop vapor diffusion method, mixxing of 0001 ml of 10 mg/mL VIM-2 protein with 0.001 ml of reservoir solution containing 0.2 M ammonium acetate, 0.1 mM zinc chloride, 0.1 M HEPES, pH 7.5, and 25% PEG 3350, X-ray diffraction tructure determination and analysis, molecular replacement Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information pre-steady-state and steady-state kinetics, overview Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ preparation of a Co(II)-substituted VIM-2 analogue, crystal structure analysis, overview Pseudomonas aeruginosa
additional information analysis of the metal content of enzyme VIM-2, metal binding kinetics, overview Pseudomonas aeruginosa
Zn2+ dependent on, crystal structure analysis, overview Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29700
-
 x * 29700, SDS-PAGE Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9K2N0 isolated from a clinical strain, gene blaVIM?2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant cobalt-cobtaining enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chromacef + H2O
-
 Pseudomonas aeruginosa (2S)-2-[(S)-carboxy(2-phenylacetamido)methyl]-5-[(E)-2-(4-nitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Pseudomonas aeruginosa (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 29700, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
metallo-beta-lactamase
-
 Pseudomonas aeruginosa
VIM-2
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing
-
 5.6