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Literature summary for 3.5.2.6 extracted from

  • Wong, W.T.; Chan, K.C.; So, P.K.; Yap, H.K.; Chung, W.H.; Leung, Y.C.; Wong, K.Y.; Zhao, Y.
    Increased structural flexibility at the active site of a fluorophore-conjugated beta-lactamase distinctively impacts its binding toward diverse cephalosporin antibiotics (2011), J. Biol. Chem., 286, 31771-31780.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Protein Variants

Protein Variants Comment Organism
E166C mutant constructed to obtain an engineered beta-lactamase that contains an environment-sensitive fluorophore conjugated near its active site to probe the structural dynamics of the omega-loop and to detect the binding of substrates. Mutant enzyme carrying a 6-bromoacetyl-2-dimethylaminonaphthalene label shows improved binding kinetics and positive fluorescence signal toward oxyimino-cephalosporins, but shows little such effect to non-oxyimino-cephalosporins. The omega-loop adopts a less stabilized structure, and readily undergoes conformational change to accommodate the binding of bulky oxyimino-cephalosporins while no such change is observed for non-oxyimino-cephalosporins Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0079
-
 penicillin G mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C Bacillus licheniformis
0.048
-
 penicillin G wild-type, pH 7.0, 22°C Bacillus licheniformis
0.072
-
 penicillin G mutant E166C, pH 7.0, 22°C Bacillus licheniformis
0.085
-
 cefuroxime wild-type, pH 7.0, 22°C Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis P00808
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cefuroxime + H2O
-
 Bacillus licheniformis (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
penicillin G + H2O
-
 Bacillus licheniformis (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
PenP
-
 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 cefuroxime wild-type, pH 7.0, 22°C Bacillus licheniformis
0.32
-
 penicillin G mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C Bacillus licheniformis
2.1
-
 penicillin G mutant E166C, pH 7.0, 22°C Bacillus licheniformis
2612
-
 penicillin G wild-type, pH 7.0, 22°C Bacillus licheniformis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 cefuroxime wild-type, pH 7.0, 22°C Bacillus licheniformis
29
-
 penicillin G mutant E166C, pH 7.0, 22°C Bacillus licheniformis
40
-
 penicillin G mutant E166C, carrying 6-bromoacetyl-2-dimethylaminonaphthalene label, pH 7.0, 22°C Bacillus licheniformis
54000
-
 penicillin G wild-type, pH 7.0, 22°C Bacillus licheniformis