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Literature summary for 3.5.2.6 extracted from
- Biochemical and genetic characterization of carbapenem-hydrolyzing beta-lactamase OXA-229 from Acinetobacter bereziniae (2012), Antimicrob. Agents Chemother., 56, 3923-3927.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Acinetobacter bereziniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00003 | - |
meropenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae |  |
| 0.029 | - |
piperacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.03 | - |
Imipenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.127 | - |
ampicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.165 | - |
cephalothin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.343 | - |
ticarcillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.405 | - |
amoxicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 1.85 | - |
Oxacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter bereziniae | I6S7G0 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.5 | - |
substrate benzylpenicillin, 30°C, pH not specified in the publication | Acinetobacter bereziniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amoxicillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ampicillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| benzylpenicillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cephalothin + H2O | - |
Acinetobacter bereziniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| imipenem + H2O | - |
Acinetobacter bereziniae | (5R)-3-[[2-(carbonoimidoylamino)ethyl]sulfanyl]-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| meropenem + H2O | - |
Acinetobacter bereziniae | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| oxacillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| piperacillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| ticarcillin + H2O | - |
Acinetobacter bereziniae | (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OXA-229 | - |
Acinetobacter bereziniae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
meropenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.09 | - |
Imipenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 0.2 | - |
cephalothin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 1 | - |
ampicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 1.7 | - |
ticarcillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 2.2 | - |
piperacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 4.2 | - |
Oxacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 11 | - |
benzylpenicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 15.2 | - |
amoxicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
meropenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 1 | - |
cephalothin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 2 | - |
Oxacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 3 | - |
Imipenem | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 5 | - |
ticarcillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 8 | - |
ampicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 38 | - |
amoxicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 80 | - |
piperacillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
| 150 | - |
benzylpenicillin | 30°C, pH not specified in the publication | Acinetobacter bereziniae | |
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