Literature summary for 3.5.2.6 extracted from

Baurin, S.; Vercheval, L.; Bouillenne, F.; Falzone, C.; Brans, A.; Jacquamet, L.; Ferrer, J.L.; Sauvage, E.; Dehareng, D.; Frere, J.M.; Charlier, P.; Galleni, M.; Kerff, F.
Critical role of tryptophan 154 for the activity and stability of class D beta-lactamases (2009), Biochemistry, 48, 11252-11263.

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, mutant enzyme W154A, mutant enzyme W154G, mutant enzyme W154H	plasmid pGEM-T-easy

Protein Variants

Protein Variants	Comment	Organism
W154F	noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10	plasmid pGEM-T-easy
W154G	noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10	plasmid pGEM-T-easy
W154H	noncarboxylated enzymes which displays poor catalytic efficiencies and reduced stability when compared to the wild-type OXA-10	plasmid pGEM-T-easy

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.004	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.005	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.005	-	cephalothin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.007	-	cephalothin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.01	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.01	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.01	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.013	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.017	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.02	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.02	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.025	-	cephalothin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.025	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.03	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.03	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.035	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.035	-	ampicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.04	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.04	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.05	-	nitrocefin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.05	-	cephaloridine	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.055	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.06	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.06	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.06	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.06	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.06	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.065	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.07	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	benzylpenicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.11	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.11	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.125	-	ampicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.125	-	Oxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.13	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.14	-	Cloxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.15	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.15	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.18	-	ampicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.2	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.2	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.2	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.21	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.21	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.22	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.23	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.25	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.25	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.29	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.4	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.45	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.45	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.6	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.65	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
2	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy

Organism

Organism	UniProt	Comment	Textmining
plasmid pGEM-T-easy	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	plasmid pGEM-T-easy

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ampicillin + H2O	-	plasmid pGEM-T-easy	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
benzylpenicillin + H2O	-	plasmid pGEM-T-easy	(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cephaloridine + H2O	-	plasmid pGEM-T-easy	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
cephalothin + H2O	-	plasmid pGEM-T-easy	(2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cloxacillin + H2O	-	plasmid pGEM-T-easy	(2R,4S)-2-[(R)-carboxy([[3-(2-chlorophenyl)-5-methyl-1,2-oxazol-4-yl]carbonyl]amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
nitrocefin + H2O	-	plasmid pGEM-T-easy	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
oxacillin + H2O	-	plasmid pGEM-T-easy	(2R,4S)-2-[(R)-carboxy[[(5-methyl-3-phenyl-1,2-oxazol-4-yl)carbonyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Subunits

Subunits	Comment	Organism
dimer	-	plasmid pGEM-T-easy

Synonyms

Synonyms	Comment	Organism
beta-lactamase OXA-10	-	plasmid pGEM-T-easy

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
39.9	-	t1/2 for mutant enzyme W154A, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
41.9	-	t1/2 for mutant enzyme W154G, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
48.8	-	t1/2 for mutant enzyme W154F, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
50.7	-	t1/2 for mutant enzyme W154G, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
53.5	-	t1/2 for mutant enzyme W154F, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
55.9	-	t1/2 for wild-type enzyme, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
56.7	-	t1/2 for mutant enzyme W154A, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
60	-	t1/2 for wild-type enzyme, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.002	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.0023	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.003	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.003	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.0036	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.004	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.012	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.0126	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.015	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.04	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.07	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.08	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.1	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.13	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.132	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.15	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.2	-	Cloxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.25	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.6	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.7	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
1	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1.3	-	cephaloridine	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
1.4	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
1.5	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1.8	-	Cloxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2	-	nitrocefin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2	-	Oxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2	-	cephalothin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
2.4	-	ampicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2.5	-	benzylpenicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2.5	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
2.5	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
2.5	-	cephalothin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
3	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
3.5	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
3.5	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
4	-	Cloxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
4	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
4	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
4	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
4.5	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
6	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
8	-	cephalothin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
8	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
18	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
35	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
35	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
70	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
85	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
90	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
120	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
120	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
160	-	ampicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
220	-	ampicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
300	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
500	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
600	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1300	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1700	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy

pI Value

Organism	Comment	pI Value Maximum	pI Value
plasmid pGEM-T-easy	OXA-10 wild-type enzyme	-	6.6
plasmid pGEM-T-easy	W154 OXA-10 mutant	-	7.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.014	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.03	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.056	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.067	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
0.25	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.3	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
0.53	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1.15	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
2	-	Cloxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
3.3	-	ampicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
4.2	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
5	-	cephalothin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
5.5	-	nitrocefin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
10	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
12	-	ampicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
12	-	Cloxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
12	-	cephaloridine	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
13	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
13	-	benzylpenicillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
13	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
14	-	cephalothin	mutant enzyme W154A, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
16	-	cephaloridine	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
16	-	Oxacillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
17.5	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
18	-	Cloxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
18	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
19	-	ampicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
20	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
23	-	cephaloridine	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
25	-	benzylpenicillin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
30	-	nitrocefin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
35	-	cephalothin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
40	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
40	-	Oxacillin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
47	-	Cloxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
53	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
57	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
80	-	benzylpenicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
100	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
120	-	ampicillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
130	-	benzylpenicillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
130	-	Oxacillin	mutant enzyme W154A, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
130	-	nitrocefin	mutant enzyme W154G, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
180	-	cephaloridine	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
190	-	nitrocefin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
320	-	cephalothin	mutant enzyme W154F, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
380	-	cephalothin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
400	-	cephalothin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
700	-	Oxacillin	mutant enzyme W154H, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
900	-	ampicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
1000	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
1500	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
2000	-	Cloxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
3000	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
3000	-	Oxacillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
6000	-	benzylpenicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
6200	-	ampicillin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy
52000	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 1.1 mM sodium bicarbonate	plasmid pGEM-T-easy
170000	-	nitrocefin	wild-type, 25°C, pH 7, in presence of 50 mM sodium bicarbonate	plasmid pGEM-T-easy