Protein Variants | Comment | Organism |
---|---|---|
additional information | lactamase CoCo-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase Cu-L1, Cu-containing analog of metallo-beta-lactamase L1. Enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase Ni-L1, Ni-containing analog of metallo-beta-lactamase L1. Enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase NiZn-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase ZnCo-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase ZnFe-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase ZnNi-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
additional information | lactamase ZnZn-L1, enzyme containing transition metal ions other than Zn(2+), prepared and characterized by kinetic and spectroscopic studies | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Imipenem | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli | |
0.008 | - |
cefaclor | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli | |
0.013 | - |
Imipenem | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.023 | - |
Imipenem | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.027 | - |
Imipenem | lactamase ZnFe-L1, 25°C, pH 7.0 | Escherichia coli | |
0.035 | - |
cefaclor | lactamase ZnFe-L1, 25°C, pH 7.0 | Escherichia coli | |
0.036 | - |
penicillin G | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.04 | - |
cefaclor | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.042 | - |
Imipenem | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
0.043 | - |
cefaclor | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.058 | - |
cefaclor | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
0.061 | - |
Imipenem | lactamase ZnNi-L1, 25°C, pH 7.0 | Escherichia coli | |
0.091 | - |
cefaclor | lactamase ZnNi-L1, 25°C, pH 7.0 | Escherichia coli | |
0.218 | - |
penicillin G | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
0.224 | - |
nitrocefin | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
0.278 | - |
penicillin G | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli | |
16 | - |
nitrocefin | lactamase NiZn-L1, 25°C, pH 7.0 | Escherichia coli | |
18 | - |
nitrocefin | lactamase Ni-L1, 25°C, pH 7.0 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | metalloenzyme | Escherichia coli | |
additional information | the metal binding sites, particularly the Zn2 site, in metallo-beta-lactamase L1 are very flexible and can accommodate a number of different divalent metal ions | Escherichia coli | |
Ni2+ | Ni2+ binds in the Zn2+-site and the ring-opened product coordinates Ni2+ | Escherichia coli | |
Zn2+ | metalloenzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cefaclor + H2O | - |
Escherichia coli | (2R)-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-5-chloro-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Escherichia coli | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | the substituted lactamases Cu-L1 and ZnNi-L1 hydrolyze cephalosporins and carbapenems, but not penicillins, suggesting that the Zn2+-site modulates substrate preference in mbetal L1 | Escherichia coli | ? | - |
? | |
nitrocefin + H2O | - |
Escherichia coli | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Escherichia coli | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mbetal L1 | - |
Escherichia coli |
metallo-beta-lactamase L1 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | nitrocefin | lactamase NiZn-L1, 25°C, pH 7.0 | Escherichia coli | |
12 | - |
Imipenem | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
13 | - |
Imipenem | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli | |
14 | - |
cefaclor | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
16 | - |
cefaclor | lactamase ZnFe-L1, 25°C, pH 7.0 | Escherichia coli | |
24 | - |
nitrocefin | lactamase Ni-L1, 25°C, pH 7.0 | Escherichia coli | |
26 | - |
cefaclor | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
27 | - |
cefaclor | lactamase ZnNi-L1, 25°C, pH 7.0 | Escherichia coli | |
29 | - |
cefaclor | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
38 | - |
cefaclor | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli | |
43 | - |
Imipenem | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
59 | - |
Imipenem | lactamase ZnFe-L1, 25°C, pH 7.0 | Escherichia coli | |
96 | - |
nitrocefin | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
118 | - |
penicillin G | lactamase CoCo-L1, 25°C, pH 7.0 | Escherichia coli | |
166 | - |
Imipenem | lactamase ZnNi-L1, 25°C, pH 7.0 | Escherichia coli | |
205 | - |
Imipenem | lactamase Cu-L1, 25°C, pH 7.0 | Escherichia coli | |
692 | - |
penicillin G | lactamase ZnCo-L1, 25°C, pH 7.0 | Escherichia coli | |
761 | - |
penicillin G | lactamase ZnZn-L1, 25°C, pH 7.0 | Escherichia coli |