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Literature summary for 3.5.2.6 extracted from

  • Meziane-Cherif, D.; Lambert, T.; Dupechez, M.; Courvalin, P.; Galimand, M.
    Genetic and biochemical characterization of OXA-63, a new class D beta lactamase from Brachyspira pilosicoli BM4442 (2008), Antimicrob. Agents Chemother., 52, 1264-1268.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLyS cells Brachyspira pilosicoli

Inhibitors

Inhibitors Comment Organism Structure
amoxicillin susceptible to amoxicillin in combination with clavulanic acid Brachyspira pilosicoli
clavulanic acid strong inhibitor Brachyspira pilosicoli
NaCl weak inhibitor Brachyspira pilosicoli
Sulbactam
-
Brachyspira pilosicoli
tazobactam strong inhibitor Brachyspira pilosicoli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
carbenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
0.019
-
benzylpenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
0.033
-
piperacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
0.043
-
ampicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
0.115
-
Oxacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
2 * 31500, SDS-PAGE Brachyspira pilosicoli
63000
-
SDS-PAGE Brachyspira pilosicoli

Organism

Organism UniProt Comment Textmining
Brachyspira pilosicoli Q50H31
-
-
Brachyspira pilosicoli BM4442 Q50H31
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Fast Flow column chromatography Brachyspira pilosicoli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Brachyspira pilosicoli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ampicillin + H2O
-
Brachyspira pilosicoli BM4442 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
benzylpenicillin + H2O
-
Brachyspira pilosicoli (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
benzylpenicillin + H2O
-
Brachyspira pilosicoli BM4442 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
carbenicillin + H2O
-
Brachyspira pilosicoli (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
carbenicillin + H2O
-
Brachyspira pilosicoli BM4442 (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
additional information does not interact with oxyimino-cephalosporins, imipenem, or aztreonam Brachyspira pilosicoli ?
-
?
additional information does not interact with oxyimino-cephalosporins, imipenem, or aztreonam Brachyspira pilosicoli BM4442 ?
-
?
oxacillin + H2O
-
Brachyspira pilosicoli (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
oxacillin + H2O
-
Brachyspira pilosicoli BM4442 (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
piperacillin + H2O
-
Brachyspira pilosicoli (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 31500, SDS-PAGE Brachyspira pilosicoli

Synonyms

Synonyms Comment Organism
OXA-63
-
Brachyspira pilosicoli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
carbenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
2.1
-
piperacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
4.9
-
benzylpenicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
6.6
-
ampicillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli
113
-
Oxacillin at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00016
-
at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli tazobactam
0.002
-
at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli clavulanic acid
0.012
-
IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli Sulbactam
2000
-
IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) Brachyspira pilosicoli NaCl