Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLyS cells | Brachyspira pilosicoli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
amoxicillin | susceptible to amoxicillin in combination with clavulanic acid | Brachyspira pilosicoli | |
clavulanic acid | strong inhibitor | Brachyspira pilosicoli | |
NaCl | weak inhibitor | Brachyspira pilosicoli | |
Sulbactam | - |
Brachyspira pilosicoli | |
tazobactam | strong inhibitor | Brachyspira pilosicoli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
carbenicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
0.019 | - |
benzylpenicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
0.033 | - |
piperacillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
0.043 | - |
ampicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
0.115 | - |
Oxacillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31500 | - |
2 * 31500, SDS-PAGE | Brachyspira pilosicoli |
63000 | - |
SDS-PAGE | Brachyspira pilosicoli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brachyspira pilosicoli | Q50H31 | - |
- |
Brachyspira pilosicoli BM4442 | Q50H31 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap Fast Flow column chromatography | Brachyspira pilosicoli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Brachyspira pilosicoli | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Brachyspira pilosicoli BM4442 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Brachyspira pilosicoli | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Brachyspira pilosicoli BM4442 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
Brachyspira pilosicoli | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
Brachyspira pilosicoli BM4442 | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
additional information | does not interact with oxyimino-cephalosporins, imipenem, or aztreonam | Brachyspira pilosicoli | ? | - |
? | |
additional information | does not interact with oxyimino-cephalosporins, imipenem, or aztreonam | Brachyspira pilosicoli BM4442 | ? | - |
? | |
oxacillin + H2O | - |
Brachyspira pilosicoli | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
oxacillin + H2O | - |
Brachyspira pilosicoli BM4442 | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Brachyspira pilosicoli | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31500, SDS-PAGE | Brachyspira pilosicoli |
Synonyms | Comment | Organism |
---|---|---|
OXA-63 | - |
Brachyspira pilosicoli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
carbenicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
2.1 | - |
piperacillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
4.9 | - |
benzylpenicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
6.6 | - |
ampicillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | |
113 | - |
Oxacillin | at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00016 | - |
at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | tazobactam | |
0.002 | - |
at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | clavulanic acid | |
0.012 | - |
IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | Sulbactam | |
2000 | - |
IC50 above 2000 mM, at 25°C in 100 mM sodium phosphate (pH 7.0) | Brachyspira pilosicoli | NaCl |