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Literature summary for 3.5.2.6 extracted from

  • Girlich, D. ; Leclercq, R. ; Naas, T.; Nordmanni, P.
    Molecular and biochemical characterization of the chromosome-encoded class A beta-lactamase BCL-1 from Bacillus clausii (2007), Antimicrob. Agents Chemother., 51, 4009-4014.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Alkalihalobacillus clausii

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid
-
 Alkalihalobacillus clausii
Sulbactam
-
 Alkalihalobacillus clausii
tazobactam
-
 Alkalihalobacillus clausii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00001
-
 cefoxitin Km less than 0.00001 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.006
-
 ticarcillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.012
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.015
-
 cephalothin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.021
-
 piperacillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.022
-
 amoxicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.035
-
 Imipenem at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.045
-
 cephaloridine at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.105
-
 cefuroxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.136
-
 cefpirome at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.79
-
 cefotaxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.95
-
 ceftriaxone at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
1
-
 ceftazidime Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
1
-
 cefepime Km above 1.0 mM at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Alkalihalobacillus clausii

Organism

Organism UniProt Comment Textmining
Alkalihalobacillus clausii A8RR46
-
-
Alkalihalobacillus clausii NR A8RR46
-
-

Purification (Commentary)

Purification (Comment) Organism
S-Sepharose column chromatography and Q-Sepharose column chromatography Alkalihalobacillus clausii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 The specific activity of purified BCL-1, measured with 0.1 mM of cephalothin as the substrate, was 35 units/mg of protein with a 26fold purification factor Alkalihalobacillus clausii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amoxicillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
aztreonam + H2O
-
 Alkalihalobacillus clausii [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
aztreonam + H2O
-
 Alkalihalobacillus clausii NR [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
benzylpenicillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Alkalihalobacillus clausii NR (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefpirome + H2O
-
 Alkalihalobacillus clausii (2S)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-5-[(6,7-dihydro-5H-cyclopenta[b]pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftazidime + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftriaxone + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[[(6-hydroxy-2-methyl-5-oxo-2,5-dihydro-1,2,4-triazin-3-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Alkalihalobacillus clausii NR (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephaloridine + H2O
-
 Alkalihalobacillus clausii (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephaloridine + H2O
-
 Alkalihalobacillus clausii NR (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalothin + H2O
-
 Alkalihalobacillus clausii (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalothin + H2O
-
 Alkalihalobacillus clausii NR (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Alkalihalobacillus clausii (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
moxalactam + H2O
-
 Alkalihalobacillus clausii (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
piperacillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ticarcillin + H2O
-
 Alkalihalobacillus clausii (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
BCL-1
-
 Alkalihalobacillus clausii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 cefoxitin kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.01
-
 moxalactam kcat less than 0.01/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.06
-
 Imipenem at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
0.3
-
 aztreonam kcat less than 0.3/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
3.3
-
 ceftazidime kcat above than 3.3/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
5
-
 ticarcillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
15
-
 cefuroxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
22
-
 cephalothin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
22
-
 cefotaxime at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
30
-
 ceftriaxone at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
38
-
 piperacillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
40
-
 cefepime kcat above 40/sec at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
85
-
 amoxicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
95
-
 cefpirome at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
97
-
 benzylpenicillin at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii
115
-
 cephaloridine at 30°C in 100 mM sodium phosphate (pH 7) Alkalihalobacillus clausii

pI Value

Organism Comment pI Value Maximum pI Value
Alkalihalobacillus clausii isoelectric focusing
-
 5.5

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000004
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii tazobactam
0.000075
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii Sulbactam
0.000085
-
 using cephalothin as a substrate, at 30°C Alkalihalobacillus clausii clavulanic acid