Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Serratia marcescens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method. Three-dimensional structures of D120A mutant and D120E mutant determined at 2.0 A and 3.0 A resolution, respectively | Serratia marcescens |
Protein Variants | Comment | Organism |
---|---|---|
D120A | kcat/KM is up to 425000fold lower than the wild-type enzyme. Ki-value for phosphate is 450fold lower than wild-type value | Serratia marcescens |
D120E | kcat/KM is up to 136000fold lower than the wild-type enzyme. the Zn-Zn distance is increased by 0.3 A compared with wild-type enzyme | Serratia marcescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive inhibition. 50 mM, 30% decrease of wild-type activity. 5 mM, 90% decrease of activity of mutant enzyme D120A | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
nitrocefin | mutant enzyme D120E | Serratia marcescens | |
0.0013 | - |
nitrocefin | mutant enzyme D120A | Serratia marcescens | |
0.008 | - |
cephaloridine | wild-type enzyme | Serratia marcescens | |
0.02 | - |
nitrocefin | wild-type enzyme | Serratia marcescens | |
0.027 | - |
nitrocefin | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.045 | - |
Imipenem | wild-type enzyme | Serratia marcescens | |
0.05 | - |
nitrocefin | mutant enzyme D120E, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.08 | - |
benzylpenicillin | wild-type enzyme | Serratia marcescens | |
0.15 | - |
cephaloridine | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.2 | - |
cephaloridine | mutant enzyme D120E | Serratia marcescens | |
0.2 | - |
cephaloridine | mutant enzyme D120E, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.27 | - |
cephaloridine | mutant enzyme D120A | Serratia marcescens | |
0.64 | - |
Imipenem | mutant enzyme D120A | Serratia marcescens | |
0.75 | - |
Imipenem | mutant enzyme D120E | Serratia marcescens | |
2.6 | - |
benzylpenicillin | mutant enzyme D120A | Serratia marcescens | |
3.1 | - |
Imipenem | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
8 | - |
benzylpenicillin | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | the Zn-Zn distance in D120E mutant is increased by 0.3 A compared with wild-type enzyme | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | P52699 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Serratia marcescens | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
Serratia marcescens | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
imipenem + H2O | - |
Serratia marcescens | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Serratia marcescens | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IMP-1 | - |
Serratia marcescens |
metallo-beta-lactamase IMP-1 | - |
Serratia marcescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
cephaloridine | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.022 | - |
cephaloridine | mutant enzyme D120E, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.17 | - |
benzylpenicillin | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
0.22 | - |
cephaloridine | mutant enzyme D120E | Serratia marcescens | |
0.23 | - |
cephaloridine | mutant enzyme D120A | Serratia marcescens | |
0.27 | - |
nitrocefin | mutant enzyme D120A | Serratia marcescens | |
0.53 | - |
benzylpenicillin | mutant enzyme D120A | Serratia marcescens | |
0.55 | - |
nitrocefin | mutant enzyme D120E | Serratia marcescens | |
2.03 | - |
Imipenem | mutant enzyme D120A | Serratia marcescens | |
3.17 | - |
Imipenem | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
3.7 | - |
Imipenem | mutant enzyme D120E | Serratia marcescens | |
4.17 | - |
nitrocefin | mutant enzyme D120E, 1 mM Zn(NO3)2 added | Serratia marcescens | |
7.5 | - |
nitrocefin | mutant enzyme D120A, 1 mM Zn(NO3)2 added | Serratia marcescens | |
90 | - |
cephaloridine | wild-type enzyme | Serratia marcescens | |
123.3 | - |
Imipenem | wild-type enzyme | Serratia marcescens | |
466.7 | - |
nitrocefin | wild-type enzyme | Serratia marcescens | |
535 | - |
nitrocefin | wild-type enzyme | Serratia marcescens | |
650 | - |
benzylpenicillin | wild-type enzyme | Serratia marcescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
phosphate | mutant enzyme D120A | Serratia marcescens | |
0.144 | - |
phosphate | wild-type value | Serratia marcescens |