Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, complexed with cefoxitin in an acyl-enzyme-adduct, for monoclinic crystals: 0.005 ml protein solution, 10 mg/ml, + equal volume of reservoir solution containing 25% PEG 6000, 0.1 M sodium acetate, pH 5.0, against 1 ml of reservoir solution at 20°C, for very big prismatic crystals: 40 mg/ml protein with lowered PEG 6000 concentration, pH 3.4, complexing by diffusion of cefoxitin for 24 h, X-ray diffraction structure determination and analysis at 1.7 A resolution | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cefoxitin | perturbs the active site structure and the conformation of the omega-loop bearing the catalytic residue Glu166, inhibition mechanism | Bacillus licheniformis | |
additional information | inhibition kinetics | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | P00808 | class A enzyme BS3 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | Glu166 is important for catalysis, mechanism | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-beta-formamidoyl-7-alpha-methoxycephalosporanic acid + H2O | - |
Bacillus licheniformis | (2R)-5-[(acetyloxy)methyl]-2-{(S)-carboxy[(E)-(hydroxymethylidene)amino]methoxymethyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
a penicillin + H2O | - |
Bacillus licheniformis | a penicilloic acid | - |
? | |
cefoxitin + H2O | - |
Bacillus licheniformis | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalotin + H2O | - |
Bacillus licheniformis | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
moxalactam + H2O | - |
Bacillus licheniformis | (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BS3 | - |
Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus licheniformis |