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Literature summary for 3.5.2.6 extracted from

  • Trehan, I.; Beadle, B.M.; Shoichet, B.K.
    Inhibition of AmpC beta-lactamase through a destabilizing interaction in the active site (2001), Biochemistry, 40, 7992-7999.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
0.98 mg/ml purified mutant N152A in complex with inhibitor moxalactam in 1.7 M potassium phosphate, pH 8.7, 23°C, after microseeding with the wild-type enzyme in 1 M potassium phosphate, pH 8.7, vapor diffusion hanging drop method, serial dilutions, 5-7 days, X-ray diffraction structure determination and analysis at 1.83 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
N152A site-directed mutagenesis, 6300fold reduced kinetic efficiency compared to the wild-type enzyme Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Imipenem beta-lactam containing a bulky substituent Escherichia coli
additional information inhibition mechanism, wild-type and mutant N152A enzyme Escherichia coli
moxalactam beta-lactam containing a bulky substituent Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P00811 class C enzyme AmpC
-
Escherichia coli JM109 P00811 class C enzyme AmpC
-

Reaction

Reaction Comment Organism Reaction ID
a beta-lactam + H2O = a substituted beta-amino acid 2-step mechanism: in the first step, the catalytic Ser64 attacks the carbonyl carbon of the beta-lactam ring, forming an acyl intermediate, in the second step, a deacylating water hydrolyzes the ester bond to regenerate the enzyme and the reaction product, hydrolyzed antibiotic Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a penicillin + H2O
-
Escherichia coli a penicilloic acid
-
?
a penicillin + H2O
-
Escherichia coli JM109 a penicilloic acid
-
?
cefalotin + H2O
-
Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefalotin + H2O
-
Escherichia coli JM109 (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
AmpC
-
Escherichia coli
serine beta-lactamase
-
Escherichia coli