Crystallization (Comment) | Organism |
---|---|
0.98 mg/ml purified mutant N152A in complex with inhibitor moxalactam in 1.7 M potassium phosphate, pH 8.7, 23°C, after microseeding with the wild-type enzyme in 1 M potassium phosphate, pH 8.7, vapor diffusion hanging drop method, serial dilutions, 5-7 days, X-ray diffraction structure determination and analysis at 1.83 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N152A | site-directed mutagenesis, 6300fold reduced kinetic efficiency compared to the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Imipenem | beta-lactam containing a bulky substituent | Escherichia coli | |
additional information | inhibition mechanism, wild-type and mutant N152A enzyme | Escherichia coli | |
moxalactam | beta-lactam containing a bulky substituent | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00811 | class C enzyme AmpC | - |
Escherichia coli JM109 | P00811 | class C enzyme AmpC | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a beta-lactam + H2O = a substituted beta-amino acid | 2-step mechanism: in the first step, the catalytic Ser64 attacks the carbonyl carbon of the beta-lactam ring, forming an acyl intermediate, in the second step, a deacylating water hydrolyzes the ester bond to regenerate the enzyme and the reaction product, hydrolyzed antibiotic | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a penicillin + H2O | - |
Escherichia coli | a penicilloic acid | - |
? | |
a penicillin + H2O | - |
Escherichia coli JM109 | a penicilloic acid | - |
? | |
cefalotin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefalotin + H2O | - |
Escherichia coli JM109 | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmpC | - |
Escherichia coli |
serine beta-lactamase | - |
Escherichia coli |