Cloned (Comment) | Organism |
---|---|
expressed in Bacillus subtilis | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
A123C | stability towards cloxacillin not enhanced | Bacillus cereus |
A77C | stability towards cloxacillin not enhanced | Bacillus cereus |
DELTA1-17 | mutant lacking the first 17 residues, first alpha helix, reduced activity, no hydrolysis of cloxacillin | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cloxacillin | hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxacillin | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
nitrocefin | - |
Bacillus cereus | |
0.065 | - |
benzylpenicillin | - |
Bacillus cereus | |
0.22 | - |
cephalosporin C | - |
Bacillus cereus | |
0.27 | - |
Cloxacillin | pH 7, 20°C | Bacillus cereus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
extracellular | - |
Bacillus cereus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus 569/H/9 | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylpenicillin + H2O | - |
Bacillus cereus | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | - |
Bacillus cereus 569/H/9 | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cephalosporin C + H2O | poor substrate, beta-lactamse I | Bacillus cereus | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
cephalosporin C + H2O | poor substrate, beta-lactamse I | Bacillus cereus 569/H/9 | N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine | - |
? | |
cloxacillin + H2O | hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin | Bacillus cereus | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cloxacillin + H2O | hydrolysis or inhibition, depending on pH, temperature, ratio enzyme/cloaxcillin | Bacillus cereus 569/H/9 | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Bacillus cereus | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Bacillus cereus 569/H/9 | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
cephalosporin C | - |
Bacillus cereus | |
34 | - |
nitrocefin | - |
Bacillus cereus | |
2200 | - |
benzylpenicillin | - |
Bacillus cereus |