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Literature summary for 3.5.2.6 extracted from

  • Mariotte-Boyer, S.; Nicolas-Chanoine, M.H.; Labia, R.
    A kinetic study of NMC-A beta-lactamase, an Ambler class A carbapenemase also hydrolysing cephamycins (1996), FEMS Microbiol. Lett., 143, 29-33.
    View publication on PubMed
Search for more literature for 3.5.2.6

Inhibitors

Inhibitors Comment Organism Structure
brobactam best inhibitory effect Enterobacter cloacae
clavulanic acid
-
 Enterobacter cloacae
tazobactam
-
 Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 meropenem
-
 Enterobacter cloacae
0.028
-
 benzylpenicillin
-
 Enterobacter cloacae
0.08
-
 cefotetan
-
 Enterobacter cloacae
0.09
-
 ceftazidime
-
 Enterobacter cloacae
0.09
-
 amoxicillin
-
 Enterobacter cloacae
0.092
-
 Imipenem
-
 Enterobacter cloacae
0.093
-
 cefoxitin
-
 Enterobacter cloacae
0.125
-
 aztreonam
-
 Enterobacter cloacae
0.152
-
 ticarcillin
-
 Enterobacter cloacae
0.185
-
 cephalothin
-
 Enterobacter cloacae
0.3
-
 moxalactam
-
 Enterobacter cloacae
0.956
-
 cefotaxime
-
 Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
 stain NOR-1
-
Enterobacter cloacae NOR-1
-
 stain NOR-1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2240
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amoxicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
amoxicillin + H2O
-
 Enterobacter cloacae NOR-1 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
aztreonam + H2O
-
 Enterobacter cloacae [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
aztreonam + H2O
-
 Enterobacter cloacae NOR-1 [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
benzylpenicillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
benzylpenicillin + H2O
-
 Enterobacter cloacae NOR-1 (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Enterobacter cloacae (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Enterobacter cloacae NOR-1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotetan + H2O
-
 Enterobacter cloacae (2R)-2-[(S)-{[4-(2-amino-1-carboxy-2-oxoethylidene)-1,3-dithietane-2-carbonyl]amino}(carboxy)methoxymethyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Enterobacter cloacae (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Enterobacter cloacae (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalothin + H2O
-
 Enterobacter cloacae (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephalothin + H2O
-
 Enterobacter cloacae NOR-1 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Enterobacter cloacae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Enterobacter cloacae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
moxalactam + H2O
-
 Enterobacter cloacae (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
ticarcillin + H2O
-
 Enterobacter cloacae (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.67
-
 ceftazidime
-
 Enterobacter cloacae
5
-
 cefoxitin
-
 Enterobacter cloacae
5
-
 cefotetan
-
 Enterobacter cloacae
8.6
-
 moxalactam
-
 Enterobacter cloacae
12
-
 meropenem
-
 Enterobacter cloacae
81
-
 ticarcillin
-
 Enterobacter cloacae
260
-
 benzylpenicillin
-
 Enterobacter cloacae
286
-
 cefotaxime
-
 Enterobacter cloacae
707
-
 aztreonam
-
 Enterobacter cloacae
816
-
 amoxicillin
-
 Enterobacter cloacae
1040
-
 Imipenem
-
 Enterobacter cloacae
2820
-
 cephalothin
-
 Enterobacter cloacae