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Literature summary for 3.5.2.6 extracted from
- High-yield expression, purification, and characterization of active, soluble Bacteroides fragilis metallo-beta-lactamase, CcrA (1997), Protein Expr. Purif., 9, 355-362.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacteroides fragilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CdCl2 | - |
Bacteroides fragilis |  |
| ZnCl2 | - |
Bacteroides fragilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
cephaloridine | - |
Bacteroides fragilis | |
| 0.009 | - |
cefotaxime | - |
Bacteroides fragilis | |
| 0.026 | - |
nitrocefin | - |
Bacteroides fragilis | |
| 0.054 | - |
cefoxitin | - |
Bacteroides fragilis | |
| 0.097 | - |
penicillin G | - |
Bacteroides fragilis | |
| 0.13 | - |
piperacillin | - |
Bacteroides fragilis | |
| 0.13 | - |
carbenicillin | - |
Bacteroides fragilis | |
| 0.2 | - |
Imipenem | - |
Bacteroides fragilis | |
| 0.25 | - |
ampicillin | - |
Bacteroides fragilis | |
| 0.34 | - |
ceftazidime | - |
Bacteroides fragilis | |
| 1.7 | - |
Methicillin | - |
Bacteroides fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25360 | - |
mass spectrometry | Bacteroides fragilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides fragilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30 | - |
nitrocefin | Bacteroides fragilis |
| 240 | - |
nitrocefin, non-fusion protein | Bacteroides fragilis |
| 260 | - |
benzylpenicillin | Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ampicillin + H2O | - |
Bacteroides fragilis | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| carbenicillin + H2O | - |
Bacteroides fragilis | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| cefotaxime + H2O | - |
Bacteroides fragilis | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| cefoxitin + H2O | - |
Bacteroides fragilis | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| ceftazidime + H2O | - |
Bacteroides fragilis | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| cephaloridine + H2O | - |
Bacteroides fragilis | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
| imipenem + H2O | - |
Bacteroides fragilis | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
| methicillin + H2O | - |
Bacteroides fragilis | (2R,4S)-2-[(R)-carboxy(2,6-dimethoxybenzamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| nitrocefin + H2O | - |
Bacteroides fragilis | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
| penicillin G + H2O | - |
Bacteroides fragilis | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
| piperacillin + H2O | - |
Bacteroides fragilis | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 25357 | Bacteroides fragilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20 | - |
cephaloridine | - |
Bacteroides fragilis | |
| 25 | - |
cefotaxime | - |
Bacteroides fragilis | |
| 75 | - |
ampicillin | - |
Bacteroides fragilis | |
| 100 | - |
Imipenem | - |
Bacteroides fragilis | |
| 100 | - |
piperacillin | - |
Bacteroides fragilis | |
| 110 | - |
penicillin G | - |
Bacteroides fragilis | |
| 140 | - |
ceftazidime | - |
Bacteroides fragilis | |
| 150 | - |
cefoxitin | - |
Bacteroides fragilis | |
| 200 | - |
nitrocefin | - |
Bacteroides fragilis | |
| 260 | - |
carbenicillin | - |
Bacteroides fragilis | |
| 350 | - |
Methicillin | - |
Bacteroides fragilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | - |
Bacteroides fragilis |
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