Literature summary for 3.5.2.6 extracted from

Rossolini, G.M.; Franceschini, N.; Riccio, M.L.; Mercuri, P.S.; Perilli, M.; Galleni, M.; Frere, J.M.; Amicosante,G.
Characterisation and sequence of the Chryseobacterium (flavobacterium) meningosepticum carbapenemase: a new molecular class B beta-lactamase showing a broad substrate profile (1998), Biochem. J., 332, 145-152.

No PubMed abstract available

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
-	Elizabethkingia meningoseptica

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Elizabethkingia meningoseptica
EDTA	-	Elizabethkingia meningoseptica
pyridine-2,6-dicarboxylic acid	-	Elizabethkingia meningoseptica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	cefoxitin	-	Elizabethkingia meningoseptica
0.029	-	cephaloridine	-	Elizabethkingia meningoseptica
0.032	-	penicillin G	-	Elizabethkingia meningoseptica
0.043	-	6-beta-iodopenicillanate	-	Elizabethkingia meningoseptica
0.066	-	nitrocefin	-	Elizabethkingia meningoseptica
0.18	-	cefotaxime	-	Elizabethkingia meningoseptica
0.37	-	Imipenem	-	Elizabethkingia meningoseptica

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Elizabethkingia meningoseptica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
26000	-	SDS-PAGE	Elizabethkingia meningoseptica

Organism

Organism	UniProt	Comment	Textmining
Elizabethkingia meningoseptica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Elizabethkingia meningoseptica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
110	-	-	Elizabethkingia meningoseptica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6-beta-iodopenicillanate + H2O	-	Elizabethkingia meningoseptica	(2R,4S)-2-[(R)-carboxy(iodo)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylate	-	?
cefotaxime + H2O	-	Elizabethkingia meningoseptica	(2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefoxitin + H2O	-	Elizabethkingia meningoseptica	(2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cephaloridine + H2O	-	Elizabethkingia meningoseptica	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
imipenem + H2O	-	Elizabethkingia meningoseptica	(5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
nitrocefin + H2O	-	Elizabethkingia meningoseptica	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
penicillin G + H2O	-	Elizabethkingia meningoseptica	(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Subunits

Subunits	Comment	Organism
monomer	1* 26000, SDS-PAGE	Elizabethkingia meningoseptica

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6	-	cefoxitin	-	Elizabethkingia meningoseptica
14	-	cephaloridine	-	Elizabethkingia meningoseptica
17	-	nitrocefin	-	Elizabethkingia meningoseptica
39	-	cefotaxime	-	Elizabethkingia meningoseptica
280	-	penicillin G	-	Elizabethkingia meningoseptica
300	-	6-beta-iodopenicillanate	-	Elizabethkingia meningoseptica
350	-	Imipenem	-	Elizabethkingia meningoseptica

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)