Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystallized without ligand and in the presence of the inhibitors 2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate and 5-fluoroorotate, hanging drop vapour diffusion method with 15-20% (w/v) polyethylene glycol 3350, 0.1 M MES (pH 6.0-6.5), 75 mM MgCl2, 150 mM KCl (with ligand) or 20-25% polyethylene glycol 3350, 0.1 M Na HEPES (pH 7) and 0.2 M NaF (without ligand) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate | - |
Escherichia coli | |
5-Fluoroorotate | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05020 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | - |
Escherichia coli | L-dihydroorotate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHOase | - |
Escherichia coli |