Protein Variants | Comment | Organism |
---|---|---|
C221S/C263S/C265S/C268S | this quadruple mutant is relatively stable to oxidation and has kinetic parameters consistent with reported values for wild type DHO | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli | |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | most active competitive inhibitor versus dihydroorotate and thio-dihydroorotate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
thio-dihydroorotate + H2O | - |
Escherichia coli | ? | - |
r |
Synonyms | Comment | Organism |
---|---|---|
DHO | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 7.0 | Escherichia coli | |
0.096 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 7.0 | Escherichia coli | |
0.12 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
0.21 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
0.32 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 9.0 | Escherichia coli | |
0.44 | - |
4,6-dioxo-piperidine-2-(S)-carboxylic acid | with dihydroorotate as substrate, pH. 9.0 | Escherichia coli | |
1.6 | - |
4(R)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
2.3 | - |
(S)-1,2,3,6-tetrahydro-6-oxopyridine-2-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli | |
3 | - |
4(S)-hydroxy-6-oxo-piperidine-2(S)-carboxylic acid | with thio-dihydroorotate as substrate, pH. 8.0 | Escherichia coli |