Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain SO1263 | Mesocricetus auratus |
expressed in Escherichia coli strain SO1263 | [Bacillus] caldolyticus |
overexpression in Escherichia coli strain BL21(DE3) | [Bacillus] caldolyticus |
overexpression in Escherichia coli strain SØ1263/pyrC- | Mesocricetus auratus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocricetus auratus | - |
- |
- |
[Bacillus] caldolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mesocricetus auratus |
- |
[Bacillus] caldolyticus |
more than 95% purified by PorosHQ column | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + H2O | - |
Mesocricetus auratus | N-carbamoyl-L-aspartate | - |
r | |
(S)-dihydroorotate + H2O | - |
[Bacillus] caldolyticus | N-carbamoyl-L-aspartate | - |
r | |
N-carbamoyl-L-aspartate | - |
Mesocricetus auratus | L-dihydroorotate + H2O | - |
r | |
N-carbamoyl-L-aspartate | - |
[Bacillus] caldolyticus | L-dihydroorotate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
BcDHOase | - |
[Bacillus] caldolyticus |
DHOase | - |
Mesocricetus auratus |
DHOase | - |
[Bacillus] caldolyticus |
hDHOase | - |
Mesocricetus auratus |
pyrC | - |
[Bacillus] caldolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | - |
Mesocricetus auratus |
25 | 45 | - |
[Bacillus] caldolyticus |
25 | 45 | differences in kinetic isotope effects due to temperature change are nonexistent for hDHOase even though both systems are run at suboptimal temperatures | Mesocricetus auratus |
37 | 60 | - |
Mesocricetus auratus |
37 | 60 | - |
[Bacillus] caldolyticus |
37 | 60 | kinetic isotope effects due to temperature change show a very moderate difference in the secondary kinetic isotope effect for BcDHOase even though both systems are run at suboptimal temperatures | [Bacillus] caldolyticus |