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Literature summary for 3.5.2.3 extracted from
- The aspartate transcarbamoylase-dihydroorotase complex in Deinococcus radiophilus has an active dihydroorotase (2006), Arch. Microbiol., 185, 78-81.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
dihydroorotase, electro-elution, SDS-PAGE and Coomassie staining | Deinococcus radiophilus |
| 498000 | - |
gel filtration, DHOase in complex with aspartate transcarbamoylase | Deinococcus radiophilus |
| 500000 | - |
aspartate transcarbamoylase-dihydroorotase complex, gel filtration | Deinococcus radiophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiophilus | - |
- |
- |
| Deinococcus radiophilus NCIMB 10648 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dihydroorotase and aspartate transcarbamoylase copurify, partially purified by anion exchange chromatography, gel filtration, hydrophobic interaction chromatography | Deinococcus radiophilus |
| HiTrap Q column chromatography, Hiload Superdex gel filtration, and phenyl-Superose HR column chromatography | Deinococcus radiophilus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl buffer, pH 8.0 | Deinococcus radiophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + H2O | aspartate transcarbamoylase-dihydroorotase complex | Deinococcus radiophilus | N-carbamoyl-L-aspartate | - |
r |  |
| (S)-dihydroorotate + H2O | aspartate transcarbamoylase-dihydroorotase complex | Deinococcus radiophilus NCIMB 10648 | N-carbamoyl-L-aspartate | - |
r | |
| L-dihydroorotate + H2O | - |
Deinococcus radiophilus | N-carbamoyl-L-aspartate | - |
? | |
| L-dihydroorotate + H2O | - |
Deinococcus radiophilus NCIMB 10648 | N-carbamoyl-L-aspartate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Deinococcus radiophilus |
| hexamer | 6 * 49000, SDS-PAGE | Deinococcus radiophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHOase | - |
Deinococcus radiophilus |
| type I DHOase | - |
Deinococcus radiophilus |
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Release 2023.1 (January 2023)
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