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Literature summary for 3.5.1.88 extracted from

  • Fieulaine, S.; Boularot, A.; Artaud, I.; Desmadril, M.; Dardel, F.; Meinnel, T.; Giglione, C.
    Trapping conformational states along ligand-binding dynamics of peptide deformylase: the impact of induced fit on enzyme catalysis (2011), PLoS Biol., 9, e1001066.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.88

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 15%-20% (w/v) PEG-3350 and either 0.1 or 0.2 M zinc acetate Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G41A the mutant shows strongly reduced Km and kcat values compared to the wild type enzyme Arabidopsis thaliana
G41M the kcat/Km value is reduced by three orders of magnitude due to large decrease in the kcat value (to 1%) compared to the wild type enzyme Arabidopsis thaliana
G41Q the kcat/Km value is reduced by three orders of magnitude due to large decrease in the kcat value (to 0.4%) compared to the wild type enzyme Arabidopsis thaliana
I130A the mutant protein exhibits strongly reduced kcat and Km values but shows no change in overall stability compared to the wild type Arabidopsis thaliana
I130F the mutant protein exhibits strongly reduced kcat and Km values but shows no change in overall stability compared to the wild type Arabidopsis thaliana
I42A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Arabidopsis thaliana
I42F the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Arabidopsis thaliana
I42N the mutant shows decreased Km and kcat values compared to the wild type enzyme Arabidopsis thaliana
I42W the mutant shows decreased Km and kcat values compared to the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
actinonin slow, tight-binding inhibitor Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FUZ2
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superdex-75 gel filtration, and Mono Q column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-formyl-Met-Ala-Ser + H2O
-
 Arabidopsis thaliana formate + Met-Ala-Ser
-
 ?

Synonyms

Synonyms Comment Organism
PDF1B
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00014
-
 actinonin at 37°C, pH not specified in the publication Arabidopsis thaliana