Literature summary for 3.5.1.88 extracted from

Dirk, L.M.; Schmidt, J.J.; Cai, Y.; Barnes, J.C.; Hanger, K.M.; Nayak, N.R.; Williams, M.A.; Grossman, R.B.; Houtz, R.L.; Rodgers, D.
Insights into substrate specificity of plant peptide deformylase, an essential enzyme with potential for the development of novel biotechnology applications in agriculture (2008), Biochem. J., 413, 417-427.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of AtPDF1Bt were grown by hanging drop vapor diffusion. The structure is determined a resolution of 2.4 A	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
Y178A	the mutant shows an increase in Km for f-MTA substrate	Arabidopsis thaliana
Y178F	mutant shows a decrease in Km as well as an increase in kcat, indicating tighter binding of the substrate	Arabidopsis thaliana

General Stability

General Stability	Organism
limited proteolysis of AtPDF1B with trypsin results in the relatively slow formation of a lower molecular mass form (AtPDF1Bt) reduced by approximately 3 kDa. Although small reductions in molecular mass are observed after prolonged incubation, the proteolyzed AtPDF1B generated was largely resistant to further digestion. Recombinant AtPDF1B has a requirement for 0.5 M NaCl for solubility at concentrations of 0.5 mg/ml. After proteolysis with trypsin, AtPDF1B loses this characteristic and is soluble without NaCl at concentrations of 30 mg/ml	Arabidopsis thaliana

Organism

limited proteolysis of AtPDF1B with trypsin results in the relatively slow formation of a lower molecular mass form (AtPDF1Bt) reduced by approximately 3 kDa. Although small reductions in molecular mass are observed after prolonged incubation, the proteolyzed AtPDF1B generated was largely resistant to further digestion. Recombinant AtPDF1B has a requirement for 0.5 M NaCl for solubility at concentrations of 0.5 mg/ml. After proteolysis with trypsin, AtPDF1B loses this characteristic and is soluble without NaCl at concentrations of 30 mg/ml

Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FV53	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-formyl-Met-Leu-p-nitroanilide + H2O	-	Arabidopsis thaliana	formate + Met-Leu-p-nitroanilide	-	?

Subunits

Subunits	Comment	Organism
dimer	AtPDF1B exists as a symmetric dimer	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtPDF1Bt	-	Arabidopsis thaliana