Literature summary for 3.5.1.88 extracted from

Kreusch, A.; Spraggon, G.; Lee, C.C.; Klock, H.; McMullan, D.; Ng, K.; Shin, T.; Vincent, J.; Warner, I.; Ericson, C.; Lesley, S.A.
Structure analysis of peptide deformylases from Streptococcus pneumoniae, Staphylococcus aureus, Thermotoga maritima and Pseudomonas aeruginosa: snapshots of the oxygen sensitivity of peptide deformylase (2003), J. Mol. Biol., 330, 309-321.

Search for more literature for 3.5.1.88

Application

Application	Comment	Organism
drug development	potential target or the development of new antibacterial agents	Plasmodium falciparum
drug development	potential target or the development of new antibacterial agents	Escherichia coli
drug development	potential target or the development of new antibacterial agents	Pseudomonas aeruginosa
drug development	potential target or the development of new antibacterial agents	Staphylococcus aureus
drug development	potential target or the development of new antibacterial agents	Streptococcus pneumoniae
drug development	potential target or the development of new antibacterial agents	Thermotoga maritima

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as His-tag fusion protein in Escherichia coli	Pseudomonas aeruginosa
expressed as His-tag fusion protein in Escherichia coli	Staphylococcus aureus
expressed as His-tag fusion protein in Escherichia coli	Streptococcus pneumoniae
expressed as His-tag fusion protein in Escherichia coli	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method	Plasmodium falciparum
sitting drop vapor diffusion method	Escherichia coli
sitting drop vapor diffusion method	Pseudomonas aeruginosa
sitting drop vapor diffusion method	Staphylococcus aureus
sitting drop vapor diffusion method	Streptococcus pneumoniae
sitting drop vapor diffusion method	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	can replace Fe2+ without loss of activity, enhances stability	Plasmodium falciparum
Co2+	can replace Fe2+ without loss of activity, enhances stability	Escherichia coli
Co2+	can replace Fe2+ without loss of activity, enhances stability	Staphylococcus aureus
Co2+	can replace Fe2+ without loss of activity, enhances stability	Streptococcus pneumoniae
Co2+	can replace Fe2+ without loss of activity, enhances stability	Thermotoga maritima
Fe2+	required, irreversible oxidation to Fe3+ results in almost complete loss of activity	Plasmodium falciparum
Fe2+	required, irreversible oxidation to Fe3+ results in almost complete loss of activity	Escherichia coli
Fe2+	required, irreversible oxidation to Fe3+ results in almost complete loss of activity	Staphylococcus aureus
Fe2+	required, irreversible oxidation to Fe3+ results in almost complete loss of activity	Streptococcus pneumoniae
Fe2+	required, irreversible oxidation to Fe3+ results in almost complete loss of activity	Thermotoga maritima
Ni2+	can replace Zn2+	Pseudomonas aeruginosa
Ni2+	can replace Fe2+ without loss of activity, enhances stability	Plasmodium falciparum
Ni2+	can replace Fe2+ without loss of activity, enhances stability	Escherichia coli
Ni2+	can replace Fe2+ without loss of activity, enhances stability	Staphylococcus aureus
Ni2+	can replace Fe2+ without loss of activity, enhances stability	Streptococcus pneumoniae
Ni2+	can replace Fe2+ without loss of activity, enhances stability	Thermotoga maritima
Zn2+	required	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-formyl-L-methionine-polypeptide + H2O	Plasmodium falciparum	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Escherichia coli	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Pseudomonas aeruginosa	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Staphylococcus aureus	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Streptococcus pneumoniae	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Thermotoga maritima	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Staphylococcus aureus ATCC 2913	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	formate + L-methionine-polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A6K3	-	-
Plasmodium falciparum	-	-	-
Pseudomonas aeruginosa	Q9I7A8	strain ATCC27853	-
Staphylococcus aureus	P68826	strain ATCC2913	-
Staphylococcus aureus ATCC 2913	P68826	strain ATCC2913	-
Streptococcus pneumoniae	Q9F2F0	strain ATCC6305	-
Thermotoga maritima	P96113	-	-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	P96113	-	-

Oxidation Stability

Oxidation Stability	Organism
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Plasmodium falciparum
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Escherichia coli
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Pseudomonas aeruginosa
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Staphylococcus aureus
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Streptococcus pneumoniae
instable towards oxidation due to the oxidation of the metal ligating cysteine residue	Thermotoga maritima

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme using His-tag	Pseudomonas aeruginosa
recombinant enzyme using His-tag	Staphylococcus aureus
recombinant enzyme using His-tag	Streptococcus pneumoniae
recombinant enzyme using His-tag	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Plasmodium falciparum	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Escherichia coli	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Pseudomonas aeruginosa	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Staphylococcus aureus	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Streptococcus pneumoniae	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Thermotoga maritima	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Staphylococcus aureus ATCC 2913	formate + L-methionine-polypeptide	-	?
N-formyl-L-methionine-polypeptide + H2O	involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	formate + L-methionine-polypeptide	-	?

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Release 2023.1 (January 2023)